极低出生体质量早产儿和足月儿外周血T淋巴细胞亚群及血清IgG水平比较

Comparison of T lymphocyte subsets and IgG level between very low-birth weight preterm children and near full term infants

探讨极低出生体质量早产儿和足月儿外周血中T淋巴细胞亚群及血清IgG水平差异。根据胎盘病理活检结果,选母亲为绒毛膜羊膜炎68例,其对应的极低出生体质量早产儿也为68例,再收集正常母亲及正常出生的婴儿20例作对照。采用流式细胞仪分别检测极低出生体质量早产儿和足月儿外周血中T细胞亚群细胞数量,同时用化学发光法测定极低出生体质量早产儿和足月儿血清中IgG含量。极低出生体质量早产儿外周血CD3^+、CD4^+、CD19^+、CD4^+/CD8^+等T细胞、NK细胞数量明显低于足月儿外周血中T细胞亚群数量,差异有统计学意义(P<0.05);而极低出生体质量早产儿外周血CD8+T细胞数量略高于足月儿外周血CD8^+T细胞数量,差异无统计学意义(P>0.05);极低出生体质量早产儿血清IgG含量明显低于足月儿血清IgG含量,差异有统计学意义(P<0.05);而且极低出生体质量早产儿CD4^+T细胞数量和胎龄呈正相关(P<0.05,r=0.619)。极低出生体质量早产儿免疫功能低下,细胞免疫在新生儿免疫中占主导地位,提高新生儿免疫功能将对降低新生儿疾病感染率和病死率具有重要的临床价值。

We aimed to explore levels of T lymphocyte subsets and lgG in very low-birth weight preterm children and near full term infants. According to the biopsy result of placenta biopsy, 68 cases of women with chorioamnionitis were chosen. All （;8 very low-birth weight preterm children were born respectively to 68 women suffered chorioamnionitis, and 20 cases of heahby infants were chosen as controls. The flow cytometry was used to determine the T cell subpopulations and chemiluminescence assay was used to detect the levels of IgG. Lymphocyte subsets of CD3＋ , CD4＋ , CD19＋ , CD4 ＋ /CD8 ＋ and NK in very low birth weight preterm children were significantly lower than those of near full term infants（P ＋ 0.05） ; and CI）8＋ subset in ver y low-birth weight preterm infants were higher than that of near full term infants, but there were no significant difference（P 〈 0.05）. The levels of IgG in very low-birth weight preterm infants differed significantly from near full term infants（P 〈 0.05）. There was a correlation between CD4＋ and fetal age in very low-birth weight preterm infants（r=0. 619, P〈0.05）. Tim im mune function was low in very low-birth weight preterm infants. Since cellular function plays an important role in neonatal, im proving neonatal immune function can significantly reduce nosoeomial infections and fatality rate, which has an important clini cal significance.