鼻咽鳞状细胞癌中PLAC8的表达及临床意义

Expressions of PLAC8 Protein in Nasopharyngeal Carcinoma Cells and Related Clinical Significance

目的探讨PLAC8在鼻咽癌组织及体外培养的鼻咽癌细胞中的表达及其与临床病理参数之间的关系。方法应用免疫组织化学染色法检测PLAC8蛋白在51例鼻咽癌组织芯片及32例鼻咽慢性炎性反应组织芯片(TMA)中的表达,分析其与鼻咽癌临床病理参数之间的关系。蛋白印迹法检测PLAC8蛋白在永生化鼻咽上皮细胞系NP69及不同鼻咽癌细胞系(CNE1、CNE2、C666-1、5-8F、6-10B)中的表达情况。结果 PLAC8在51例鼻咽癌组织中表达主要定位于细胞质,表达率为72.5%,在32例鼻咽慢性炎性反应组织中表达率为46.9%,两者间差异有统计学意义(P<0.01)。在鼻咽癌组织中PLAC8的表达与T分期、临床分期及远处转移无关(P>0.05),而与淋巴结转移有关(P<0.05)。蛋白印迹法发现PLAC8蛋白在多株鼻咽癌细胞系中的表达相对于人永生化鼻咽上皮细胞系NP69的表达明显升高。结论 PLAC8可能在鼻咽癌的发生、发展过程中起着重要作用,并可能调控了鼻咽癌的淋巴结转移。

Objective To investigate the expression and role of PLAC8 protein in nasopharyngeal carcinoma（NPC） tissues and NPC cells cultured in vitro, and their relationship with clinicopathological features. Methods The expressions of PLAC8 protein in 51 cases of NPC tissues and 32 cases of chronic inflammation of nasal pharynx tissues （tissue microarray, TMA） were detected by immunohistochemical staining. The relationship of PLAC8 expressions with the clinical and pathological features of NPC was analyzed. The expressions of PLAC8 in NPC cells （CNEI, CNE2, C666-1, 5-8F and 6-10B） and immortalized nasopharyngeal epithelial cell line NP69 were also detected by Western blot. Results The expression of PLAC8 in NPC cells was mainly localized in the cytoplasm. The positive expressions of PLAC8 was 72.5% in 51 cases of NPC tissues and 46.9% in 32 cases of chronic inflammation of nasal pharynx tissues（P〈0.01）. The positive expression of PLAC8 in NPC cells was significantly associated with lymph node metastasis （P〈0.05）, not T stage, clinical stage or distant metastasis （P〉0.05）. Compared with NP69 cells, the expression of PLAC8 was positive in multi-strain nasopharyngeal carcinoma cell lines. Conclusion PLAC8 may play an imoortant role in the develooment of NPC. It mac regulate lymph node metastasis of NPC.