利用响应面优化固态发酵生产毛叶山桐子脱脂果渣发酵饲料

Optimization of response surface for fermented feed in Idesia polycarpa residues through solid-state fermentation

文章利用响应面法对毛叶山桐子发酵饲料的发酵条件进行了优化.实验菌种采用了产朊假丝酵母,实验材料为毛叶山桐子的脱脂果渣.首先利用单因素实验对五个影响发酵的指标进行了研究,然后在单因素的基础上利用Plackett-Burman实验设计筛选出其中显著的三个因子并利用Box-Behnken响应面设计对其进行优化.优化后的条件为发酵温度30℃,料液比1∶1.2,接种量10.5%,硝酸铵添加量8.8%,发酵时间4d.经优化条件发酵后,毛叶山桐子脱脂果渣发酵饲料的粗蛋白由发酵前的8.49%提升到19.13%,脱脂果渣的营养组成变得更加合理.

In this study, response surface methodology （RSM） was performed the optimization of the fermentation of Idesia polycarpa residues, and Candida utilis was selected for solid-state fermenta- tion. Five influences of the fermentation were studied by one-factor tests, and the Plackett-Burman ex- periment subsequent confirmed three main influences for Box-Behnken RSM design. Optimized condi- tions of the fermentation as follow： residues with ratio of solid to liquid 1 ： 1.2, NH4NOa additive a- mount 8.8%, and Candida utilis inoculation 10.5% fermented in 30℃ for 4 days. Fermentation results showed that, the crude protein content of fermented feed increased from 8.49% to 19.13%, and the nu- trition ratio of products became more reasonable. This study suggests that Idesia polycarpa residues have a real potential on the modern farming by the solid-state fermentation.