摘要
目的测定SPF级C1型尼曼-匹克病小鼠(Npc1^(-/-)小鼠)的生长繁殖及血液生理生化指标,为开展NPC1病人的研究提供理论性的基础资料。方法 (1)选取0~77日龄Npc1^(-/-)、Npc1^(+/-)和Npc1+/+小鼠雌雄各40只,定期称重并绘制生长曲线;(2)Npc1^(-/-)小鼠由Npc1^(+/-)小鼠交配繁殖产生,统计Npc1^(+/-)小鼠连续4代的繁殖数据;(3)检测60日龄Npc1^(-/-)和Npc1+/+小鼠的血液生理生化指标。结果 (1)Npc1^(-/-)小鼠的体重在7周前随着日龄的增加而增加,7周后随着日龄的增加而减少,直至11周左右死亡。Npc1+/+和Npc1^(+/-)小鼠的体重均随着日龄的增长而逐渐增加,两者之间并没有显著差别。4周后雄性比雌性体重增加明显比雌性小鼠快;(2)Npc1^(+/-)小鼠不同代内交配分娩间隔、窝产仔数、离乳仔数、离乳仔数中雌雄数量及阳性数量差异无显著性(P>0.05),而第2代的离乳率明显大于第1代(P<0.05);(3)Npc1^(-/-)和Npc1+/+小鼠血液生理指标中平均红细胞血红蛋白含量(MCH)和平均过氧化物酶指数(MPXI)差异有显著性(P<0.05),其余差异无显著性(P>0.05)。生化指标中尿素(UREA)差异有极显著性(P<0.01),而天门冬氨酸氨基转移酶(AST)、葡萄糖(GLU)、乳酸脱氢酶(LDH)、钾(K)和铜(Cu)等差异有显著性(P<0.05),其余差异无显著性(P>0.05)。结论 (1)Npc1^(-/-)、Npc1^(+/-)和Npc1+/+小鼠的生长曲线因基因型和性别的不同而存在差异;(2)Npc1^(+/-)小鼠不同代的繁殖能力差异无显著性;(3)Npc1^(-/-)和Npc1+/+小鼠的部分血液生理生化指标差异有显著性。
Objective To investigate the growth curve,breeding rate,and blood physiological and biochemical parameters in Npc1 gene mutant mice( Npc1-/-) for providing theoretical evidence in research on Niemann-Pick disease type C1( NPC1) patient. Methods 1) The body mass of Npc1-/-,Npc1+/-,and Npc1+/+mice( n = 120; 60♀,60♂) was measured from 0 to 77 days;( 2) As Npc1-/-mice were born only by the mating Npc1+/-mice,the breeding rate of Npc1+/-mice was counted here from the 1st to 4th generation;( 3) The blood physiological and biochemical parameters were measured on both Npc1-/-and Npc1+/+mice at 60 days. Results 1) Compared with the wild type controls,thebody weight of Npc1-/-mice was progressively increased up to 7 weeks and then decreased,and died around 11 weeks.The body weight of the Npc1+/-and Npc1+/+mice was increased as time went on. After 4 weeks,the male mice showed a higher weight gain than the females;( 2) The generations of Npc1+/-mice had no significant difference in mating-parturition interval,litter size,weaning litter and the number of male and female( P 〉 0. 05),but the weaning rate of the 2nd generation was significantly higher than that of the 1st generation( P 〈 0. 05);( 3) The hematological parameters showed a significant difference only in mean corpuscular hemoglobin( MCH) and mean peroxidase index( MPXI) between the Npc1-/-and Npc1+/+mice( P 〈 0. 05). No significant difference was found in other hematological parameters( P 〉0. 05). Among the biochemical parameters,aspartate aminotransferase( AST),glucose( GLU),lactate dehydrogenase( LDH),potassium( K) and copper( Cu) had a significant difference between the Npc1-/-and Npc1+/+mice( P 〈0. 05). Conclusions 1) The growth curves of Npc1-/-,Npc1+/-,and Npc1+/+mice are different due to different genotype and sex;( 2) The reproduction rates of Npc1+/-mice have no significant difference among different generations;( 3)The blood physiological parameters( MCH,MPXI) and biochemical parameters( UREA,AST,GLU,LDH,K,Cu) are significantly different between Npc1-/-and Npc1+/+mice.
出处
《中国实验动物学报》
CSCD
北大核心
2017年第3期250-255,共6页
Acta Laboratorium Animalis Scientia Sinica
基金
国家自然科学基金项目(编号:81400936
81600987
U1304808)
河南省高等学校重点科研项目(编号:16A180014)
河南省高校科技创新人才支持计划(14HASTIT032)
河南省科技发展计划项目(142300410192)
新乡医学院精神神经医学学科群支持计划
关键词
C1型尼曼-匹克病
小鼠
生长曲线
繁殖
血液参数
Niemann-Pick disease type C1,Mice
Growth rate
Reproduction
Hematological parameters