30亿PIB/mL甘蓝夜蛾核多角体病毒悬浮剂的研制

Development of Mamestra Brassicae Nucleopolyhedrovirus(MabrNPV) 3.0×10~9 PIB/mL Suspension Concentrate

本文通过对润湿分散剂、增稠剂、防冻剂、防腐剂和消泡剂等助剂的筛选,确定了最佳制剂配方为:甘蓝夜蛾核多角体病毒30亿PIB/m L,农乳600#P磷酸酯3.0%,NNO 2.0%,白炭黑1.0%,黄原胶0.05%,硅酸镁铝1.0%,丙三醇5.0%,苯甲酸钠0.3%,异辛醇1.0%,纯水补足至100%。通过湿式超微粉碎法加工工艺制成悬浮剂,结果表明,该配方制备的悬浮剂分散性好,悬浮率≥90%,热贮和冷贮稳定性合格,其他各项质量控制指标均符合要求。

In this paper, the suspension concentrate was prepared by optimizing the main adjuvants such as wetting-dispersing agents, thickening agents, antifrezing agents, preservative as well as defoaming agents, and the optimum formula was determined as follows： MabrNPV（3.0×10^9 PIB/mL）, agricultural emulsifier 600# phosphate （3.0 %）, NNO （2.0 %）, white carbon bLack （1.0 %）, xanthan gum （0.05 %）, magnesium alumino silicate （1.0 %）, glycerol （5.0 %）, sodium benzoate （0.3 %）, Isooctyl alcohol （1.0 %） and deionized water making up to 100 %. The suspension was prepared by wet ultrafme grinding process. It was showed that the suspension agent prepared with good dispersibility, and the suspension rate was over 90 %. All of them reached the quality standard of suspension concentrate.