春石斛杂交种与药用金钗石斛3种活性成分的比较研究

Comparative study of three kinds of active ingredients between Nobile-type Dendrobium and Dendrobium nobile Lindl.

比较分析了春石斛杂交种Dendrobium Second Love‘Tokimeki’与传统药用石斛金钗石斛活性成分,结果显示,D.Second Love‘Tokimeki’9月至翌年1月的茎多糖含量均值高于金钗石斛;多糖DHPP-Ⅰ数均分子量和重均分子量均低于金钗石斛多糖DNPP-Ⅰ;两种石斛茎都含有所检测的19种氨基酸,金钗石斛所测的氨基酸总量高于D.Second Love ‘Tokimeki’,但D.Second Love ‘Tokimeki’的8种必需氨基酸总量高于金钗石斛;金钗石斛的Fe和B元素含量显著高于D.Second Love ‘Tokimeki’,而D.Second Love ‘Tokimeki’的Mn、Zn、Cu元素含量显著高于金钗石斛。通过两种石斛3种活性成分比较可知,D.Second Love ‘Tokimeki’多糖含量较高,但多糖DHPP-Ⅰ数均分子量和重均分子量均低于金钗石斛多糖DNPP-Ⅰ;D.Second Love ‘Tokimeki’的氨基酸、矿质元素与金钗石斛组成相同,但含量有所差异。

This paper analyzed the active ingredients contents betwen Dendrobium Second Love ‘Tokimeki＇and the traditional medicinal D. nobile to provide reference for the development of D. Second Love‘Tokimeki＇. The content of polysaccharide was determined by phenol sulfuric acid colorimetry,and the molecular weight of polysaccharides was detected by Gel Permeation Chromatography. The content of free amino acid was determined by LC-MS/MS. The mineral element content was determined by Graphite furnace atomic absorption spectrophotometer 240ZAA（GTA120Z）. Results showed that the mean value of polysaccharide content of D. Second Love ‘Tokimeki＇ was higher than that of D. nobile from September to January of next year,the number-average molecular weight and weight- average molecular weight of DHPP-Ⅰ of D. Second Love ‘Tokimeki＇ were lower than DNPP-Ⅰ of D. Nobile. There were 19 kinds of free amino acids measured in both species of Dendrobiums,the total free amino acid content of D. nobile was higher than that of D. Second Love ‘Tokimeki＇,but the total content of eight kinds of essential amino acids in D. Second Love‘ Tokimeki＇ was higher than that of D. Nobile. The content of Fe and B elements of D. nobile were higher than those of D. Second Love‘Tokimeki＇,but the content of Mn,Zn and Cu elements of D. Second Love‘ Tokimeki＇ were higher than those of D. Nobile. By comparative analysis of three kinds of active ingredients in both species of Dendrobiums,we can know that the polysaccharide content of D. Second Love ‘Tokimeki＇ was higher,and the number-average molecular weight and weight-average molecular weight of DHPP-Ⅰ of D. Second Love‘Tokimeki＇ were lower than DNPP-Ⅰ of D. Nobile,the composition of free amino acids and mineral element in both species of Dendrobiums were the same,but the contents were different. The results could provide a reference for the medicinal development of D. Second Love‘Tokimeki＇ .