广州白云机场口岸入境海鱼感染异尖线虫鉴定研究

Identification on the infection of Anisakis from marine fishes imported by Baiyun Airport

对2012—2015年从白云机场口岸入境的214批海鱼进行剖检鉴定异尖线虫,取内脏和鱼肉进行酶消化,在10批次海鱼中分离出虫体,用苯酚乳酸透明液进行透明处理及形态学鉴定,确定所分离出的虫体有异尖线虫三期幼虫Ⅰ型和Ⅱ型。用线虫rDNA ITS区通用引物NC5和NC2对虫体进行PCR扩增,并对虫体的核糖体基因间隔区ITS1和ITS2进行序列和进化分析,确定分离出来的异尖线虫三期幼虫包括简单异尖线虫(Anisakia simple)、抹香鲸异尖线虫(Anisakia physeteris)和典型异尖线虫(Anisakia typica)。调查结果对公共安全的风险评估有重要意义,同时为口岸的进境水产品食品安全风险评估提供参考数据。

214 batches of marine fish imported in 2012-2015 from Baiyun Airport in Guangzhou were examined for Anisakis larvae by dissection and digest. 10 batches of Anisakis larvae were isolated from marine fishes by enzymatic degradation. Some Anisakis larvae isolated from imported marine fish were identified via morphology as the third larvae of Anisakis Type Ⅰ and the rest were identified as the third larvae of Anisakis Type Ⅱ. Sequencing and phylogenetic analysis of the ribosomal internal transcribed spacer （ITS） gene identified nematodes as A. simplex,A. physeteris and A. typica. The study has an important impact on the public health risk assessment,and also provides the reference for food safety risk assessment of imported marine fish and aquatic products.