TAZ与胰腺癌细胞增殖和抗凋亡能力的关系研究

Relationship of TAZ with proliferation and anti apoptotic ability of pancreatic cancer

目的探讨转录共激活因子TAZ对胰腺癌细胞增殖与抗凋亡能力的影响及其作用机制,为胰腺癌寻找新的诊断与治疗靶点提供思路。方法用空载病毒(对照组)、TAZ过表达病毒(Lv-TAZ)和TAZ干扰病毒(siTAZ)转染胰腺癌细胞后用CCK8法检测各组细胞的增殖情况;流式细胞仪检测各组细胞的凋亡率;Western blotting检测各组细胞的PTEN、Akt、p-Akt的表达情况。结果与对照组相比,Lv-TAZ组细胞增殖率升高,siTAZ组细胞增殖率下降。与对照组相比,Lv-TAZ组细胞凋亡率下降,siTAZ组细胞凋亡率升高。Western blotting结果显示,Lv-TAZ组细胞PTEN表达水平下调,p-Akt上调;siTAZ组细胞PTEN表达水平上调,p-Akt下调。结论 TAZ在胰腺癌细胞中可能通过调控PTEN/Akt信号通路发挥其促增殖和抗凋亡作用。

Objective To investigate the effect and functionary mechanism of TAZ on the proliferation and anti apoptotic ability of pancreatic cancer cells,and to provide a new way for the diagnosis and treatment of pancreatic cancer.Methods Pancreatic cancer cells were transfected with lentiviral vector expressing TAZ（Lv-TAZ）and TAZ siRNA lentiviral vector（siTAZ）,and then CCK8 was used to detect the proliferation.Flow cytometry was used to detect the apoptosis.Western blotting was used to detect the expressions of PTEN,Akt,p-Akt.Results Compared with the control group,the proliferation rate of cells transfected with Lv-TAZ was increased,and the cell proliferation rate of cells transfected with siTAZ was decreased.Compared with control group,the apoptosis rate of cells transfected with Lv-TAZ was decreased,and the apoptosis rate of cells transfected with siTAZ was increased.Western blotting results showed that the expression of PTEN in cells transfected with Lv-TAZ was downregulated,and the expression of p-Akt was upregulated.On the contrary,the expression of PTEN in cells transfected with siTAZ was upregulated,and the expression of p-Akt was downregulated.Conclusion TAZ may play a role in promoting the proliferation and anti apoptosis by regulating PTEN/Akt signaling pathway in pancreatic cancer cells.