胡萝卜苷抑制HepG-2细胞增殖和迁移及拓扑异构酶表达

Daucosterol inhibits the proliferation and migration of HepG2 cells and topoisomerase expression

目的探讨胡萝卜苷对HepG2细胞增殖、迁移及DNA拓扑异构酶(topoisomerase,TOPO)Ⅰ和Ⅱ表达的影响。方法体外培养人肝癌HepG2细胞。CCK-8实验观察不同浓度(200μg/ml、150μg/ml、100μg/ml和50μg/ml)胡萝卜苷对HepG2细胞增殖的影响。qRT-PCR实验观察200μg/ml胡萝卜苷对HepG2细胞TOPOⅠ和TOPOⅡmRNA表达的影响。Transwell小室模型检测200μg/ml胡萝卜苷对HepG2细胞迁移的影响。结果胡萝卜苷对HepG2细胞增殖有明显抑制作用,并在一定范围内(0μg/ml^200μg/ml)呈现浓度依赖性。与未用胡萝卜苷处理的HepG2比较,经200μg/ml胡萝卜苷作用的HepG2细胞穿越基膜的数量显著减少,TOPOⅠ和TOPOⅡmRNA相对表达量显著降低。结论胡萝卜苷可能通过下调TOPOⅠ、TOPOⅡ基因表达而抑制HepG2细胞增殖,并具有抑制HepG2细胞迁移的能力。

Objective To study the effect of daucosterol intervention on the proliferation and migration of HepG2 cells and the expression of DNA topoisomerase （TOPO） I and II , Methods Human hepatocellular carcinoma HepG2 cells were resuscitated and cultured in vitro. CCK-8 experiment was conducted to observe the effect of different concentrations （200μLg/ml, 150μg/ml, 100μLg/ml, 50μg/ml） of daucosterol on the proliferation of HepG2 cells. Transwell chamber models were established to detect the effects of 200μg/ml daucosterol on the migration of HepG2 cells. The effect of 200μg/ml daucosterol on the expression of TOPO I mRNA and TOPO II mRNA in HepG2 cells was observed through RT-qPCR experiment. Results Daucosterol significantly inhibited the prolifera-tion of HepG2 cells in vitro, in a concentration-dependent manner within a certain range （0μg/ml-200μg/ml）. The number of HepG2 cells migrating through the basilemma was significantly less in the group treated with 200μg/ml daucosterol than in the group without daucosterol intervention; the expression of related TOPO I mRNA and TOPO II mRNA was also much lower. Conclusion Daucosterol may inhibit the proliferation and migration of HepG2 cells through the down-regulation of TOPO I and TOPO II expression.