血管内皮生长因子-D在乳腺癌淋巴转移中的临床意义

The clinical significance of vascular endothelial growth factor-D in lymphatic metastasis of breast cancer

目的探讨乳腺癌中血管内皮生长因子-D(VEGF-D)对淋巴管生成的作用及其与临床病理指标和预后的关系。方法应用免疫组织化学S-P法检测120例乳腺癌组织中VEGF-D的免疫反应性,同时以D2-40、podoplanin标记微淋巴管,计数微淋巴管密度(LVD),分析其与临床病理指标及预后的关系。结果乳腺癌组织中VEGF-D蛋白免疫反应阳性率、LVD值均明显高于乳腺纤维腺瘤组织;乳腺癌组织中VEGF-D蛋白免疫反应与淋巴结转移呈正相关,且LVD值与VEGF-D表达呈线性相关。结论 VEGF-D参与乳腺癌淋巴管生成,影响乳腺癌的预后。

Objective To investigate the role of vascular endothelial growth factor-D （VEGF-D） in the lymphangiogenesis of breast cancer and to evaluate its relationship with the clinicopathological characteristics and prognosis. Methods The S-P immunohisto- chemical method was used to detect VEGF-D immunoreactivity in 120 cases of breast cancer, and lymphatic endothelial cells were marked with D2AQ and podoplanin simultaneously. Lymphatic vessel density （LVD） was then counted, and its relationship with the clinical pathological parameters and prognosis of breast cancer was analyzed. Results Both the positive rates of VEGF-D and LVD were significantly higher in breast cancer than in breast fibro-adenoma tissue. VEGF-D immunoreactivity was positively associated with the lymph node metastasis of breast cancer. LVD was linearly correlated with the expression of VEGF-D. Conclusion VEGF-D affects breast cancer prognosis through facilitating lymphangiogenesis.