骆驼肠道内3种含氮杂环化合物富集培养的微生物多样性

Diversity of Microbe in the Intestinal Tract of Camelus bactrianus Enriched by Three Kinds of Nitrogen Heterocyclic Compounds

为研究骆驼(Camelus bactrianus)体内是否存在潜在的杂环化合物降解菌,采集3头骆驼肠道内容物,分别以吡啶、喹啉和吲哚3种含氮杂环化合物作为唯一碳源和氮源进行5代富集培养.通过高通量测序技术对肠道内容物和5代富集培养微生物进行了测序分析.结果显示:骆驼肠道中经过杂环化合物(吡啶、喹啉、吲哚)富集后,变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、浮霉菌门(Planctomycetes)、拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes)5个门类在富集的微生物中丰度最高;骆驼肠道内吡啶富集的优势菌属于芽孢杆菌属(Bacillus)、鞘氨醇杆菌属(Sphingo-bacterium)、苍白杆菌属(Ochrobactrum)和假单胞菌属(Pseudomonas),吲哚富集的优势菌属于芽孢杆菌属、棒状杆菌属(Corynebacterium)和赖氨酸芽孢杆菌属(Lysinibacillus),而喹啉富集的优势菌以鞘氨醇杆菌属为主.骆驼肠道内容物原始样品经过吡啶、喹啉、吲哚富集5代后,优势菌群发生了较大的改变,这暗示骆驼肠道内存在具有降解吡啶、吲哚和喹啉潜能的微生物,且涉及这3种杂环化合物的降解菌群不同.

To explore the biodiversity of bacteria degrading potentially heterocyclic compounds in the intestinal tract of camel（Ca-melus bactrianus）,we took pyridine,quinolone and indole as the only carbon and nitrogen resources,and used the five generations of enrichment culture method to cultivate the camel rumen bacteria,and used high-throughput sequencing（Illumina Miseq）to sequence the total DNA of the five generations.The results showed that Proteobacteria,Actinobacteria,Planctomycetes,Bacteroidetes and Firmicutes constituted the five categories of bacteria with the highest abundance in the intestinal tract of camel which degraded heterocyclic compounds.The dominant bacteria for degrading pyridine belong to Bacillus,Sphingobacterium,Ochrobactrum and Pseudomonas,for degrading quinolone belong to Bacillus,Corynebacterium and Lysinibacillus,and for degrading indole belong to Sphingobacterium,respectively.However,compared with the original samples in the intestinal tract,the dominant bacteria groups enriched in the cultures of five generations obviously changed.This suggest that the intestinal tract of camel has the bacteria degrading heterocyclic compounds,and the involved species are different for degradation of pyridine,quinolone and indole.