人参花蕾对D-半乳糖大鼠骨代谢的影响

Effects of ginseng flower bud on bone metabolism in rats induced by D-galactose

目的建立D-半乳糖致♂大鼠骨丢失动物模型,采用骨形态计量学方法、生物力学等观察人参花蕾对大鼠骨组织的影响,并探讨其作用机制。方法 3月龄的清洁级♂SD大鼠53只,随机分为5组,其中正常组13只,正常组颈背部皮下注射生理盐水5 m L·kg^(-1)·d^(-1),其余组颈背部皮下注射D-半乳糖100 mg·kg^(-1)·d^(-1)。正常组(NS)和模型组(DG)给予溶剂对照,其余组分别予:阳性药物组(司坦唑醇0.54 mg·kg^(-1)·d^(-1)+吡拉西坦432 mg·kg^(-1)·d^(-1),CP)、低剂量人参花蕾组GF(L)(0.486 g·kg^(-1)·d^(-1))、高剂量人参花蕾组GF(H)(2.43 g·kg^(-1)·d^(-1)),均灌胃给药,连续14周。测量胫骨上段松质骨和胫骨中段皮质骨骨组织形态计量学参数;检测大鼠右侧股骨(三点弯曲法)的生物力学性能,检测左侧股骨骨钙、磷以及骨胶原的含量。结果与DG组比较,低、高剂量的人参花蕾分别使胫骨上段的骨小梁数量增加,骨小梁分离度减少,每毫米破骨细胞数和破骨细胞贴壁长度减少。与DG组比较,低、高剂量人参花蕾组胫骨中段的皮质面积百分率增加、骨髓面积百分数下降。与DG组比较,低、高剂量人参花蕾组股骨BWW/BW明显降低,弹性载荷增加。结论低、高剂量的人参花蕾能有效对抗D-半乳糖所致的大鼠胫骨上段和中段骨的丢失,机制与抑制破骨细胞的骨吸收有关,但对股骨无机质丢失的预防作用不明显。

Aim To investigate the skeletal effects of ginseng flower bud（ GF） on osteopenia induced by Dgalactose using histomorphometry and biomechanical properties. Methods Fifty three-month-old male Sprague-Dawley rats were randomly divided into five groups. Rats in NS group（ NS） were treated with NS（ 5m L·kg-1·d-1） by subcutaneous injection and daily oral gavage with vehicle as control. Rats in the other four groups were given D-galactose at the dose of 100 mg · kg-1· d-1by subcutaneous injection. Solvent control was performed between NS and DG： gastric irrigation with distilled water of 10 m L · kg-1· d-1.Other groups were： CP was gastric irrigated with integrated medicine（ stanozolol 0. 54 mg·kg-1·d-1＋ piracetam 432 mg· kg-1·d-1）,GF（ L） with ginseng flower bud of 0. 486 g · kg-1·d-1and GF（ H） with ginseng flower bud of 2. 43 g · kg-1· d-1for 14 weeks. The longitudinal proximal tibial metaphyseal（ PTM）,the fifth lumbar vertebral body（ LVB） and tibial shaft（ Tx） sections were performed undecalcifiedly and used for bone histomorphometric analysis. 858Mini Bionix materials testing system was used to analyze the biomechanic properties of right femur via three-point bending test. The left femur was dried and assimilated,whose bone calcium（ Ca）,phosphate（ P）content and bone hydroxyproline content were tested.Results Compared with D-glagatose group,in PTM of D-galactose treated rats,the % Tb. Ar was increased both in GF（ L） and GF（ H） treated groups. While the Tb. Sp was decreased. % Oc. S. Pm and Oc. N/mm decreased in GF（ L）,and those in GF（ H） were decreased as well. In Tx,% Ct. Ar was raised,while %Ma. Ar was decreased in GF（ L） and GF（ H）. The elastic load of femur was increased. Conclusions Compared with DG group,there are significant differences in bone histomorphometry of Tx and PTM in all doses of GF, but no significant changes are detected in hydroxyproline,Ca,and P content of femur.