核仁素对小鼠糖尿病性心肌病心肌肥大与纤维化发生的影响

Role of nucleolin on myocardial hypertrophy and fibrosis in type Ⅱ diabetic cardiomyopathy mice

目的:阐明核仁素在小鼠糖尿病性心肌病中的作用。方法:前期研究中我们采用了心肌特异性过表达核仁素转基因小鼠制备了Ⅱ型糖尿病性心肌病模型;实验分为野生型对照组、转基因对照组、野生型糖尿病组及转基因糖尿病组。采用麦胚凝集素(wheat germ agglutinin,WGA)荧光染色检测小鼠心肌肥大情况;采用Masson三色染色检测小鼠纤维化情况;采用Power Lab system检测小鼠心肌功能变化。结果:与对照组比较,转基因糖尿病组小鼠的血糖值变化不明显,心肌细胞肥大显著减轻,胶原纤维生成显著减少,血流动力学指标±dp/dtmax、左室舒张末压、左室峰压和心率均有不同程度的改善,且差异具有统计学意义。结论:核仁素可能通过减轻糖尿病性心肌病小鼠心肌细胞肥大及纤维化的发生,从而改善糖尿病性心肌病小鼠的心功能。

AIM ： To investigate the effect of nucleolin on diabetic cardiomyopathy in mice. METHODS ： Atype Ⅱ diabetic cardiomyopathy mouse model was prepared using a cardiac-specific nucleolin-overexpressing transgenic mice. The mice were divided into wild-type mouse control group, nucleolin transgenic mouse control group, wild-type mouse diabetes group and nucleolin transgenic mouse diabetes group. Wheat germ agglutinin （WGA） fluorescent dye, Masson staining and PowerLab system detection were used to further clarify the role of nucleolin on cardiac hypertrophy, fi-brosis and cardiac function in type Ⅱ diabetic cardiomyopathy mice. RESULTS ： Compared with wild-type mouse control group, no significant increase in blood glucose level was found, while genetical myocardial cell hypertrophy was significant-ly attenuated in nucleolin transgenic mouse diabetes group. The collagen fibers were also significantly reduced, and hemo-dynamic indexes ± dp/dtmax,left ventricular end-diastolic pressure,left ventricular systolic pressure and heart rate were also improved. The above differences were statistically significant. CONCLUSION ： Nucleolin may reduce the occurrence of myocardial hypertrophy and fibrosis, thus improving the cardiac function of diabetic cardiomyopathy mice.