中国西沙群岛沐浴海绵的化学成分研究

Chemical constituents fromSpongia sp.,a marine sponge in Xisha Islands

目的研究中国西沙群岛沐浴海绵Spongiasp.的化学成分。方法运用正相硅胶柱色谱、反相ODS柱色谱、Sephadex LH-20凝胶柱色谱以及半制备高效液相色谱等多种色谱学分离手段,对沐浴海绵Spongiasp.的石油醚萃取层进行分离纯化;通过理化性质、波谱学数据并结合文献报道鉴定化合物的结构,采用微量稀释法评价化合物的体外抗真菌活性。结果从海绵Spongiasp.中分离并鉴定了9个化合物,分别为smenodiol(1)、smenospongorine(2)、5-epi-smenospongorine(3)、dictyoceratin C(4)、epi-smenospongidine(5)、dictyoceratin A(6)、stigmasta-4,6,8(14),22-tetraen-3-one(7)、3-oxo-4,6,8(14)-triunsaturated steroid(8)、ergosta-4,6,8(14),22-tetraen-3-one(9)。结论化合物1~9均为首次从该属海绵中分离得到,体外抗真菌活性测试显示,化合物2、3、5和9对3种受试菌株(白色念珠菌、须癣毛癣菌和红色毛癣菌)表现出程度不等的抑制活性,MIC值范围在12.5~25μg/ml。

Objective To investigate the chemical constituents in the marine sponge , Spongia sp .,collected from the Xisha Islands .Methods The pure chemical components from the petroleum ether extract of Spongia sp .were obtained by re-peated column chromatography on silica gel ,ODS ,Sephadex LH-20 and semi-preparative HPLC .Their structures were deter-mined by spectroscopic analysis and comparison with the reported data .The antifungal activity of those compounds was evalua- ted by dilution method .Results 9 compounds were isolated and identified ,including smenodiol （1） ,smenospongorine（2 ）,5-epi- smenospongorine（3）, dictyoceratin C（4）, e_p-smenospongidine（5）, dictyoceratin A （ 6 ）, stigmasta-4, 6, 8（14）, 22-tetrae『3-one （7）, 3-oxo-4, 6 , 8（14）-triunsaturated steroids （8）, ergosta-4, 6 , 8（14）, 22-tetraen-3-one（9） .Conclusion Compounds 1？ 9 were isolated from the sponge of genus s_pongia for the first time .Compound 2、 3 、 5 and 9 exhibited antifungal activities against Can-dida albicans,Trichophyton mentaqro_phytes and Trichophyton rubrum with the MIC values of 12 .5-25 μg /ml.