不同栽培方式对金线莲中微量元素的影响

Effects of Different Cultivation Methods on Trace Elements in Anoectochilus roxburghii(Wall.) Lindl

[目的]建立微波消解-ICP-MS法测定金线莲中微量元素,为企业种植金线莲及质量标准的制定提供依据。[方法]样品微波消解后,采用ICP-MS法测定各元素含量,比较不同品种、不同栽培方式及月龄对金线莲中各微量元素的影响。[结果]各微量元素标液工作曲线的线性良好,相关系数≥0.999,精密度RSD≤4.4%,重复性RSD≤5.8%,回收率89.4%~114.2%,回收率RSD≤4.8%。金线莲不同品种间微量元素含量有较大差异,其中以Ca、Mg、Fe、Mn、Zn的含量较高。[结论]金线莲中的微量元素含量随种植周期的延长而增长。该方法快捷准确、灵敏稳定、重现性好,可用于同时测定金线莲中各微量元素的含量。

[Objective]The research aimed to establish the microwave digestion inductively coupled plasma mass spectrometry （ICP-MS） method for content determination of trace elements in A.roxburghii,which provided the basis for the development of A.roxburghii and the quality standard.[Method]After the treatment of microwave digestion, ICP-MS method was used in the determination.The effects of different cultivars, different cultivation methods and age on the trace elements of A.roxburghii were studied.[Result]The standard liquid working curves of trace elements had good linearity, with the correlation coefficients of more than 0.999.The relative standard deviation（RSD） of precision was less than 4.4% and RSD of repeatability was less than 5.8%.The average recovery was 89.4%-114.2%,with RSD of less than 4.8%.Different varieties of A.roxburghii presented great differences in contents of inorganic elements, with high content of Ca, Mg, Fe, Mn, Zn.[Conclusion]The content of trace elements increase along with the prolonged cultivation cycle.The method is fast and accurate, sensitive and stable, good reproducibility.It can be used for the simultaneous determination of trace elements in A.roxburghii.