茶多酚对猪精液常温保存效果的影响

Effects of tea polyphenol on boar sperm quality during room temperature storage

【目的】研究茶多酚(TPP)对猪精液常温保存效果的影响。【方法】手握法采集20月龄健康杜洛克种公猪精液,在基础稀释液中添加不同质量浓度的TPP(0(CK),0.01,0.02,0.04,0.08,0.16g/L)作为稀释液进行常温(17℃)保存,每隔24h检查精子活率、质膜完整率和顶体完整率,分别在精液保存0,2,4,6d时检测精液中的细菌含量,并在3d和6d时检测精液中的丙二醛含量(MDA)和总抗氧化(T-AOC)活性。【结果】添加0.02,0.04,0.08g/L TPP可明显提高猪精子活率、质膜完整率、顶体完整率和T-AOC活性,降低精液的MDA含量和细菌含量。且以0.04g/L TPP效果最佳,精液有效保存时间为6d。6d时0.04g/L TPP组猪精子活率、质膜完整率、顶体完整率、丙二醛(MDA)含量、总抗氧化(T-AOC)活性和细菌含量分别为60.02%,46.40%,75.25%,2.39nmol/L,2.31U/mL和2.19×106 CFU/mL。【结论】茶多酚能有效提高猪精液常温保存效果,最适添加量为0.04g/L。

[Objective] This study aimed to investigate the effects of tea polyphenol （TPP） on room temperature storage of boar sperm. [Method] Semen samples were collected from 20 months old health Du- roc boars with massage method, and diluted with basic liquid containing different concentrations of TPP （0（CK） ,0.01,0.02,0.04,0.08 and 0.16 g/L）. Sperm motility, plasma membrane integrity and acrosome integrity were measured every 24 h, bacterial content were measured at 0,2,4, and 6 d, and malondialde- hyde （MDA） content and total antioxidative capacity （T-AOC） were measured at 3 and 6 d during the stor- age at 17 ℃. [Result] Basic liquid supplemented with TPP at concentrations of 0. 02,0. 04,and 0.08 g/L significantly improved sperm motility, plasma membrane integrity, and acrosome integrity obviously, while decreased MDA and bacterial contents in boar semen （P〈0.05）. The 0.04 g/L TPP treatment had the op- timum effect, and the effective storage time of sperm was 6 days. After 6 days, sperm motility, plasma mem- brane integrity,acrosome integrity, MDA content,and bacterial content were 60.02% ,46.40% ,75.25%, 2.39 nmol/L, 2.31 U/mL, and 2.19 × 106 CFU/mL, respectively. [Conclusion] TPP could improve quality of boar semen during room temperature storage, and 0.04 g/L TPP was the optimum concentration.