肢体缺血后处理对大鼠局灶性脑缺血再灌注损伤保护作用的研究

Research of neuroprotective effect of the remote limb ischemic postconditioning in rats local ischemia reperfusion injury

目的:探讨肢体缺血后处理对大鼠脑缺血再灌注损伤的神经保护作用。方法:用线栓法制备SD大鼠局部脑缺血模型,随机分为假手术组、缺血再灌注组和肢体缺血后处理组(n=12)。缺血再灌注后24 h分别进行神经功能评分、TTC染色、血浆SOD、MDA水平检测。结果:与缺血再灌注组比较,肢体缺血后处理组神经功能评分降低、第二脑片梗死体积缩小、血浆MDA水平降低(P均<0.05)。结论:肢体缺血后处理可改善大鼠脑缺血再灌注后神经功能,减小梗死体积,减低氧化应激水平。

Objective： To explore the neuroprotective effect of the limb ischemic postconditioning on rats ischemia reperfusion injury. Methods： To prepare middle cerebral artery occlusion model with thread,the SD rat were randomly divided into sham operation group,ischemia reperfusion group and limb ischemic postconditioning group（n = 12）. After ischemia reperfusiong 24 h,nerve function was assessed,cerebral infarct volume was measured by TTC staining,plasma SOD activity and MDA were measured. Results：Compared with ischemia reperfusion group,rat neurobehavioral score was lower,the cerebral infarct volume was significantly smaller,plasma MDA contents was lower in limb ischemic postconditioning group（P〈0.05）. Conclusion： Limb ischemic postconditioning has a protective effect against rat cerebral ischemia reperfusion injury,can improve neurological function,decrease cerebral infarct volume and reduce level of oxidative stress.