酶法检测游离脂肪酸试剂盒的稳定性研究

Stablility research of non esterified fatty acids diagnostic reagent

目的:评价游离脂肪酸测定试剂盒(酶法)的稳定性。方法:采用奥林巴斯AU680全自动生化分析仪,通过重复性、线性试验测定试剂盒的稳定性。结果:两个水平质控中物质浓度的变异系数(CV)无论在0时间还是各种加速试验开瓶20 d后都小于5%,在0.1~3.0 mmol/L浓度范围内线性良好。结论:上海复星长征医学科学有限公司研发的游离脂肪酸测定试剂盒稳定、结果准确,操作快速、简便,可满足大批量临床样本的常规和自动化分析。

Objective： To evaluate the stability of non esterified fatty acids diagnostic reagent. Methods：The content of NEFA were determined with Olympus AU680 automatic biochemical analyzer by repeatability and linearity tests. Results： The coefficients of variation（CV） of the contents at two levels（0-20 d） of quality control were less than 5%. The determination of linear samples showed a good linearity over the range of 0. 1 - 3 mmol/L. Conclusion： The NEFA reagent made by Shanghai Fuxing company can provide a simple,quick,accurate and stable method for the determination of non esterified fatty acids in the clinical serum or plasma samples,can meet the requirement of the conventional and automated analysis of mass clinical samples.