不同提取方法对江南卷柏中双黄酮含量的影响及抗氧化活性评价

Effects of Different Extraction Methods on Content of Biflavonoids in Selaginella moellendorfii and Evaluation of Its Antioxidant Activities

采用超临界流体萃取法、快速溶剂萃取法、回流法、微波法、超声法和温浸法对江南卷柏中双黄酮类化合物进行提取,以穗花杉双黄酮作为标准物质,应用高效液相色谱考察不同溶剂提取物中双黄酮的含量。此外,采用3种不同的体外抗氧化方法,评价不同方法提取物对不同自由基的清除效果。结果表明:江南卷柏的回流提取物中穗花杉双黄酮的含量最高;不同方法的提取物均具有清除DPPH·、O_2^-和OH·的能力,且清除能力与提取物溶液浓度存在量效关系,回流提取物清除各自由基能力较强。

In order to evaluate the performances of the optimal extraction method,six extraction methods including accelerated solvent extraction,SFE-CO_2 extraction,reflux extraction,Microwave extraction,Ultrasonic extraction and Soaking extraction were used to extract bioactive compounds from S. moellendorfii. HPLC method was attempted for quantitative analysis and comparison among different extracts was made by determining the content of amentoflavone. Furthermore,antioxidative activity of different extracts were determined by capacity of scavenging DPPH ·,O_2^-and OH·.The results indicate that extraction temperature has significant effect on extraction efficiency,and reflux extraction is proved to the effective technique for extracting biflavonoid from S. moellendorfii;DPPH·,O_2^-and OH· were scavenged by extracts of S. moellendorfii,and scavenging capacity increases with the content of extracts; Compared among different extracts,antioxidant activities of reflux method was the highest.