过量表达水稻EPFL家族基因影响拟南芥气孔的发育

The Effects for Stomata Developmental on Arabidopsis by Overexpression of Rice EPFL Family Genes

气孔由两个保卫细胞围合而成是植物重要的与外界气孔交换的器官,其在发育过程中受到环境与内部因素的控制,表皮原母细胞由一次不对称分裂开始经过一系列分化,最终形成2个保卫细胞并形成气孔。最近在对EPFL家族基因(epidermis patenting factor like,EPFLs)研究中,发现EPFLs编码的小肽可能在气孔发育中起到调节作用,特别是对气孔密度及在气孔系细胞发生与发展都有调节作用。本研究利用生物信息学方法,比对了水稻(Oryza sativa)基因组,找到与拟南芥(Arabidopsis thaliana)同源的水稻EPFLs基因,构建载体转化拟南芥,以拟南芥为表型研究对象,研究水稻EPFLs在调控气孔形成过程中可能起到的作用,研究发现水稻EPFLs也具有调节气孔发育的作用,其中与Stomagen同源的Os01g0914400水稻基因的表型与拟南芥Stomagen表型非常相似,过表达都增加了气孔的数量与密度,水稻EPFLs中有3个基因Os04g0637-300、Os03g0672500、Os04g0457700转化拟南芥后表现气孔密度显著减少,研究说明水稻中也有调节气孔发育的相关基因,可能对水稻气孔发育与分布密度有影响作用。

Stomata consists of two guard cells that function as turgor-operated valves, regulate gas exchange in plants. The development of stomata has interaction with inner regulations and environmental factors, an asymmetric division in the epidermis of plants initiates a lineage that ultimately produces stomatal guard cells. Our research focused on the EPFL（epidermis patenting factor like） family genes of rice（Oryza sativa）, which aliment was made by using bioinformatics approach. We found that Os01g0914400 of rice which is homologous gene with Stomagen of Arabidopsis, has same phenotypes as Stomagen by transformed with over-expressed vector on Arabidopsis, and three genes of rice EPFLs genes, Os04g0637300、Os03g0672500 and 0s04g0457700, exhibited reduced stomatal density, it shown there should have same genes, which have functions on regulation of stomatal differentiation and density distribution in rice.