浙江省三地区冷鲜鸡中大肠埃希菌耐药谱测定及MLST分子分型分析

Drug resistance spectrum analysis and MLST genotyping of Escherichia coli from refrigerated chicken in three regions of Zhejiang Province

大肠埃希菌是食品中普遍污染的条件致病菌,其耐药性非常严重,耐药基因具有极大的传播风险。为了解浙江省地区禽源大肠埃希菌耐药性特点及分布规律,试验从浙江省3个地区市售冷鲜鸡中分离了59株大肠埃希菌并测定了耐药谱,发现有24种耐药谱,显示了分离株耐药类型的多样性。同时对分离株进行了Multilocus sequence typing(MLST)分子分型,获得38个已知ST型,并发现2个新的ST型。在此基础上,针对7个看家基因进行了系统发育分析,分离株显示了一定的地区差异性。该试验结果可为浙江省禽源大肠埃希菌的耐药性评价和溯源分析提供参考依据。

Escherichia coli is a common opportunistic pathogen in food. Its drug resistance is severe in which the related resistance genes can easily spread. In this study,59 strains of E. coli from refrigerated chicken in three regions of Zhejiang Province were isolated. Using drug resistant spectrum determination,24 types of drug resistant spectrum were found,which showed that the drug resistant types of E. coli isolates were multifarious. Meanwhile,all the E.coli isolates were analyzed with MLST genotyping. The results showed that a total of 38 ST types were obtained,and two new ST types were found as well. On this basis,the phylogenetic analysis of 7 housekeeping genes showed a certain degree of the regional differences. This research results can provide scientific reference for the evaluation of drug resistance and traceability analysis of avian Escherichia coli in Zhejiang province,and a wider region in China.