大熊猫阴道源乳酸杆菌生物学特性研究

Study on biological characteristics of Lactobacillus from giant panda vagina

用大熊猫阴道源的3株乳酸杆菌(Lactobacillus salivarius GPV01、Lactobacillus plantarum GPV02和Lactobacillus saerimneri GPV03)作为试验组,用人阴道源3株乳酸杆菌(Lactobacillus acidophilus WAV01、Lactobacillus crispatus WAV02和Lactobacillus plantarum WAV03)作为对照组,进行产过氧化氢能力、耐酸性、耐热性、抗菌活性、药物敏感性及黏附性研究。结果表明:在相同的培养条件下,L.salivarius GPV01、L.plantarum GPV02和L.saerimneri GPV03的产H2O2能力和耐热能力不及L.acidophilus WAV01、L.mcrispatus WAV02和L.plantarum WAV03。L.salivarius GPV01、L.plantarum GPV02和L.plantarum WAV03具有很好的酸耐性,在培养基pH为2~3之间时,3株菌的D值在0.2~0.55之间,其中L.plantarum GPV02的D值最高,达到0.55。同时,6株乳酸杆菌的培养上清液对大肠埃希菌、金黄色葡萄球菌和溶血性链球菌均有不同程度的抑制作用,6株乳酸杆菌对3种致病菌的抑制作用远弱于pH 3.5的乳酸。6株乳酸杆菌对多西环素、氨苄西林、头孢曲松、头孢噻肟、阿莫西林高度敏感,而对四环素和利福平一般敏感。6株乳酸杆菌对链霉素、氧氟沙星、庆大霉素、复方新诺明、环丙沙星、磺胺甲基异恶唑表现出耐药。6株乳酸杆菌均能黏附到Hela细胞上,GPV01、03,WAV01、03对Hela细胞的平均黏附数为18.8~32.8·cell^(-1)。WAV02黏附能力最强(54.75±2.40)个·cell^(-1),而GPV02黏附能力最弱(6.83±0.33)·cell^(-1)。

In the present study, 3 Lactobacillus strains of giant panda vagina {Lactobacillus salivarius GPV01, Lacto-bacillus plantarum GPV02 and Lactobacillus saerimneri GPV03） were used as experimental group, and 3 Lactobacil-lus strains of woman vagina （Lactobacillus acidophilus WAV01, Lactobacillus crispatus WAV02 and Lactobacillus plantarum WAV03） were used as control group to explore the difference of these strains in hydrogen peroxide produc-tion capacity, acid resistance, heat resistance, antibacterial activity, drug sensitivity and adhesiveness. It was shown that L. acidophilus WAV01 , L. crispatus WAV02 and L. plantarum WAV03 possessed higher H2 0 2-producing abili-ty and heat-resistance than L. salivarius GPV01 , L. plantarum GPV02 and L. saerimneri GPV03 under the same cul-ture condition. L. salivarius GPV01, L. plantarum GPV02 and L. plantarum WAV03 exhibited acid tolerance. D values of Lactobacillus suspensions were all between 0. 2 to 0. 55 for these strains when the pH was between 2 to 3 in the culture medium, and among these strains, L. plantarum GPV02 showed the highest D value （D = 0. 55） under the above condition. Six culture supernatants of the Lactobacillus strains had different inhibitory effects on Escherichia coli, Staphylococcus aureus and Hemolytic streptococcus. Six Lactobacillus strains showed weaker inhibition on three pathogenic bacteria than lactic acid （ pH 3. 5）. Six Lactobacillus strains were highly sensitive to doxycycline, ampi- cillin, ceftriaxone, cefotaxime, amoxicillin, and were sensitive to tetracycline and rifampicin. Six Lactobacillus strains showed resistance to streptomycin, ofloxacin, gentamycin, selectrin, ciprofloxacin and sulfamethoxazole. Six Lactobacillus strains could stick on Hela, and the average adhesion number of GPV01 , GPV03 , WAV01 and WAV03 on each Hela cell was 18. 8 - 32. 8. WAV02 had the strongest adhesion ability （54. 75 ± 2. 40） ？ cell-1,while GPV02 had the worst adhesion ability （6. 83 ±0. 33） .cell-1.