苹果YABBY基因家族的鉴定、进化及表达分析

Identification,evolution and expression analysis of the YABBY gene family in apple(Malus×domestica Borkh.)

从苹果全基因组范围内共鉴定出13个YABBY基因,通过聚类分析将其分为YAB1/YAB3、YAB2、INO、YAB5和CRC 5个亚家族。Md YABBY基因分布在苹果的9条染色体上,在第16条染色体上分布最多。Md YABBY蛋白长度介于60~538个氨基酸,等电点为4.84~9.81,所有Md YABBY蛋白都包含有锌指结构和YABBY两个保守结构域,同一个亚家族内的成员表现出相似的基因结构和蛋白保守基序分布;基因顺式作用元件分析发现,Md YABBY基因包含许多与抗逆和激素响应有关的作用元件。基因表达分析发现,Md YABBY基因在花、果和叶中的表达量较高,5个Md YABBY基因在果实发育的3个时期均明显地上调表达。

13 Malus × domestica YABBY genes were characterized and divided into five groups （ YAB1/YAB3, YAB2, INO, YAB5 and CRC）. All the M. domestica YABBY genes distributed on nine chromosomes in which the sixteenth contained the maximum amount of members. The length and isoelectric points of M. domestica YABBY pro-teins were from 60 to 538 amino acids and 4. 84 to 9. 81, respectively. All the 13 M. domestica YABBY proteins had a zinc finger-like domain in the N terminus and a YABBY domain in the C terminus, and the members of one sub-group present similar gene structure and conserved protein motifs, then the cw-elements analysis found that there were many cw-elements related to stress and hormone response. Gene expression analysis indicated that most of the M. do-mestica YABBY genes were highly expressed in flower, fruits and leaves. Five MdYABBY genes were all greatly up- regulated during three fruit development.