长链非编码RNA LINC00672与肺癌预后的关联研究

Long non-coding RNA LINC00672 contributes a favorite survival of lung cancer

目的探索长链非编码RNA LINC00672在肺癌组织中的表达及其与患者预后的关系。方法采用实时荧光定量PCR技术检测LINC00672在75对肺癌组织和癌旁正常组织中的表达,分析其在癌组织中的表达水平与肺癌患者临床分期和预后的关联。结果 LINC00672在肺癌组织中的表达显著低于癌旁正常组织(P=0.026),LINC00672高表达与低表达相比能显著降低肺癌患者的死亡率(死亡风险比=0.46;95%置信区间=0.23~0.95;P=0.036),延长患者中位生存期(34个月vs 18个月,P=0.027)。并且,LINC00672与肺癌预后的关联在低年龄组(<60 a)、吸烟者和非饮酒者中更为显著。进一步相乘交互作用分析显示LINCOO672与饮酒在肺癌死亡风险上具有显著的交互效应(P=0.049)。然而,LINC00672的表达水平在不同分期、T、N、M患者来源的肺癌组织中的表达无显著性差异。结论 LINC00672与肺癌发生发展存在关联,可用于预测肺癌患者的预后。

Objective To explore the expression status of long non-coding RNA LINC00672 in lung cancer tissues and its correlation with survival of lung cancer. Methods We applied the real-time PCR method to measure the expression level of LINC00672 in 53 pairs of lung cancer tissues and adjacent lung normal tissues,and analyzed the correlation between its expression and survival of lung cancer. Results LINC00672 was significantly down-regulated in lung cancer tissues than their adjacent lung normal tissues（P = 0. 026）. Compared to those with low expression level of LINC00672,patients with high expression level of LINC00672 exerted a significant long median survival time than those with low expression level（34 vs18 months,P = 0. 027）. High LINC00672 expression also contributed to low mortality rate than low LINC00672 expression（hazard ratio = 0. 46,95% confidence interval = 0. 23-0. 95,P = 0. 036）. Meanwhile,the correlation was more evident in those low age groups（〈 60 years）,smokers and non-drinkers. There was also a significant interaction between LINC00672 and drinking on affecting death risk of lung cancer. However,no significant association was observed between LINC00672 expression and clinical stages as well as T,N,M status. Conclusion LINC00672 is correlated with development of lung cancer,which may be a valuable biomarker to predict lung cancer prognosis.