摘要
定向进化技术是近二十年发展起来的蛋白质改造技术,其可通过对基因的突变和重组来构建突变库,然后通过高通量的筛选鉴定性能改善的突变体,此技术是改善酶性能最有效的途径之一。本文就定向进化技术的几种常用方法和定向进化后的文库筛选、应用及展望进行了概述。
Directed evolution method has been a kind of new technology of protein modification developed in recent 20 years,which build mutant library through the gene mutation and restructuring,and then identified the performance improvement of mutants with high-throughput screening methods.This was the effective way to improve enzyme properties.This paper reviewed the several frequently-used methods of directed evolution and library screening after directed evolution of protein.In addition,the application of recombination for directed evolution and prospection for the future of this technology also described.
出处
《中国饲料》
北大核心
2017年第14期15-19,23,共6页
China Feed
基金
国家自然科学基金(31360562
31160467
30960256
31260543)
关键词
定向进化
易错PCR
DNA改组
定向筛选
directed evolution
error prone PCR
DNA shuffling
directed screening