不同处理诱导新海16号体细胞胚胎同步化发生

Synchronized Somatic Embryogenesis of 'Xinhai 16' Induced by Different Treatments

【目的】旨在探索诱导海岛棉体细胞胚胎同步化发生的有效方法。【方法】以海岛棉品种新海16号的胚性愈伤组织为材料,研究了低温、饥饿和渗透处理诱导体细胞胚胎同步化发生的效果。【结果】在低温处理组中,4℃诱导时体细胞胚胎发生的数量随着低温诱导时间的延长而显著增加;10℃诱导时,不同诱导时间下各处理体细胞胚胎发生的数量差异不显著,但都显著高于对照(28℃);缺磷、缺氮、缺肌醇3种饥饿处理的体细胞胚胎的数量都随着处理时间的延长而减少;渗透处理组中,在含40 g·L-1葡萄糖的培养基上诱导7 d的效果最佳。【结论】4℃处理3 d、缺磷处理5 d、缺氮处理5 d以及40 g·L-1葡萄糖诱导7 d可以显著促进新海16号的体细胞胚胎同步化发生,其中4℃处理3 d的诱导效果最佳。

[Objective] The aim of this study was to develop an efficient method for synchronizing the somatic embryogenesis of Gossypium barbadense L. [Method] We attempted to synchronize the somatic embryogenesis of G. barbadense L. cv. ＇Xinhai 16＇ embryonic calli. The somatic embryos induced by low temperature, starvation, and osmotic pressure were counted and analyzed. [Result] In the low-temperature treatment group, the number of somatic embryos increased significantly at 4 ℃. While, there were no significant differences in the number of somatic embryos between treatment times at 10 ℃. Nevertheless, more somatic embryos were produced at 10 ℃ than at 28 ℃ （control temperature）. Somatic embryogenesis was inhibited by the nutrient starvation treatment （i.e., phosphate, nitrogen, or inositol deficiency）. In the osmotic stress group, the best results were observed following a 7-day culture in medium containing 40 g·L^-1 glucose. [Conclusion] ＇Xinhai 16＇ somatic embryogenesis can be synchronized by incubating calli at 4 ℃ for 3 days, simulating phosphate or nitrogen starvation conditions for 5 days, or maintaining calli in a medium containing 40 g·L^-1 glucose for 7 days. The low-temperature treatment may provide optimal synchronization conditions.