16种中草药对牛源致病性大肠杆菌的体外抑菌效果

Antibacterial effects of Chinese herbal medicines on bovine pathogenic Escherichia coli in vitro

为研究中草药对5种血清型牛源致病性大肠杆菌(Escherichia coli O_1、E.coli O_2、E.coli O_8、E.coli O_(78)和E.coli O_(86))的体外抑菌效果,通过牛津杯法测定中草药对致病性大肠杆菌的抑菌圈直径,通过微量稀释法和平板法测定最小抑菌浓度(MIC)和最低杀菌浓度(MBC)。结果表明:1)金银花和黄连对E.coli O1体外抑菌效果最好,抑菌圈直径分别为29.35和37.71mm,五味子对E.coli O8体外抑菌效果最好,抑菌圈直径为28.97mm。2)金银花和黄莲对E.coli O_1的MIC分别为0.016和0.008g/mL,五味子对E.coli O8的MIC为0.008g/mL,抑菌效果较好。3)虎杖的体外抑菌效果最差,没有检测出抑菌圈,MIC和MBC。结果显示金银花、黄连和五味子对5种血清型大肠杆菌的抑菌圈直径均大于15mm达到极敏,地榆、穿心莲、虎杖、柴胡、黄柏和石榴皮不敏感,没有检测出抑菌圈。研究发现金银花、黄连、何首乌、地榆、五味子和秦皮对大肠杆菌抑菌效果较好。

The aim of this study was to study the antibacterial effects of 16 Chinese herbal medicines on 5different serotypes of pathogenic Escherichia coli（O1、O2、O8、O78and O86）in vitro.The diameter of inhibition zone was determined by Oxford cup method.Micro dilution and plate method were determined by minimal inhibitory concentration（MIC）and minimum bactericidal concentration（MBC）.1）Lonicera japonica and Coptis chinensis had the best inhibitory effects on E.coli O1,and the inhibition zones were 29.35 and 37.71 mm respectively.Schisandra chinensis had the best inhibitory effect on E.coli O8 the inhibition zone was 28.97 mm.2）The MIC of L.japonica and C.chinensis on E.coli O1 were 0.016 and 0.008g/mL respectively.The MIC of S.chinensis on E.coli O8 was 0.008g/mL.All herbs displayed better inhibitory effects.3）Polygonum cuspidatum had the worst inhibitory effects,inhibition zone,MIC and MBC were not detected.The inhibition zone of L.japonica,C.chinensis and S.chinensis on 5different serotypes were above 15 mm and reached significantly sensitive,Radix sanguisorbae,Andrographic,P.cuspidatum,R.bupleuri,golden cypress and Pomegranate husk were not sensitive.L.japonica,C.chinensis,P.multiflorum,R.sanguisorbae,S.chinensis and Cortex fraxini displayed better inhibitory effects on 5different serotypes.