哮喘患者体内血清淀粉样蛋白P与循环纤维细胞含量相关性分析

Correlation of Serum Amyloid P Levels and Circulating Fibrocytes Percentages in Asthma Patients

目的:探讨循环纤维细胞、血清淀粉样蛋白P(SAP)在哮喘发病中的作用及其相关性。方法:收集健康志愿者和哮喘患者外周血,分别用流式细胞仪检测单个核细胞中循环纤维细胞百分比,ELISA法检测血清中SAP浓度,并对哮喘患者上述两个指标进行相关分析。结果:哮喘患者体内循环纤维细胞(CD45+ColⅠ+细胞)的百分比显著高于健康志愿者,而SAP浓度显著低于健康志愿者,在哮喘患者,两者呈负相关(r=-0.566,P<0.05)。结论:哮喘患者体内循环纤维细胞比例上调,而SAP浓度下调,且两者之间呈负相关,提示SAP可能作为哮喘气道重塑的一个生物学标志。

Objective：To investigate the potential role of serum amyloid P（SAP）on circulating fibrocytes（CFs）in asthma patients.Methods：11healthy controls and 15 asthma patients were recruited and informed consented.Peripheral blood mononuclear cells were isolated and circulating fibrocytes（CD45＋ColⅠ＋cells）percentages were analyzed with flow cytometry.Levels of SAP in serum were analyzed with ELISA.The correlation of SAP levels and circulating fibrocytes percentages were analyzed.Results：Elevated frequency of circulating fibrocytes and decreased SAP concentration were detected in asthma patients compared with those in normal controls.There was an inverse correlation of circulating fibrocytes frequency and SAP concentration in asthma patients（r=-0.566,P〈0.05）.Conclusion：In asthma patients,decreased SAP concentration might be the cause of increased number of circulating fibrocytes,and may play a role in airway remodeling of asthma.