两种大鼠哮喘模型建立方法的比较

Comparison of Two Methods to Establish Rat Models of Asthma

以两种方法建立哮喘大鼠模型,方法一:用新鲜配制的卵清蛋白(OVA)(0.2 mg)致敏,氢氧化铝作为佐剂,连续7天进行1%OVA雾化激发,30 min/次;方法二:用高剂量卵清蛋白(2 mg)致敏,氢氧化铝(Al(OH)_3)与百日咳灭活杆菌共同作为佐剂,先以1.7%OVA气管滴注染毒,随后1%OVA连续雾化激发6 d,30 min/次。通过肺组织形态学观察、无创肺功能测试、肺泡灌洗液(BALF)中炎性细胞分类及计数、组织样本中特异性蛋白IgE以及卵清蛋白特异性IgE(OVA-IgE)含量作为敏感指标判断哮喘模型建立成功与否。结果显示两种哮喘模型大鼠均表现出哮喘大鼠的肺组织病理学改变,肺功能降低,BALF中的细胞总数、淋巴细胞、嗜酸性粒细胞数增加;采用方法二能够引起组织样本中IgE以及OVA-IgE含量增高,而采用造模方法一未观察到此类变化。因此,方法二能够更好地制备过敏性哮喘模型,可作为较好的哮喘大鼠模型建立方法。

Rats allergic asthma models were estabalished in two different ways. The first one was sensitized with ovatbumin （OVA） （0.2 mg） freshly prepared, A1 （OH）3 used as an adjuvant and 1% OVA atomized for seven days, 30 minutes a time; The second one was sensitized by high dose ovalbumin （2 mg）, AI（OH）3 used as an adjuvant with Bordetella pertussis, and first exposed by 1.7% OVA instillation, followed by 1% OVA continuous nebulization for 6 days, 30 minutes a time. To determine whether the asthma model is successful or not, the tistopathological changes of lung tissue and noninvasive pulmonary function were detected; bronchoalveolar lavage fluid （BALF） was collected to count the number of inflammatory cells; the homogenate supernatant of lung tissue was collected for detecting the concentrations of immunoglobulin E （IgE） and Ovalbumin immunoglobulin E （OVA-IgE）. The results showed that, in the two asthmatic rats, the lung tissue pathological was changed; the lung function were decreased; the total number of ceils and the number of lymphocytes and eosinophils in BALF were increased. The second method could increase the content of IgE and OVA-IgE in lung tissue, but the first one was not observed. So the second method to make asthma model is better, thus it could be choosen in the following studies.