长链非编码RNA-HOTAIR对子宫内膜癌转移侵袭能力的影响

Influence of long chain non-coding RNA( lncRNA)-HOTAIR on migration and invasion ability of endometrial cancer

目的探讨长链非编码RNA(lncRNA)-HOTAIR对子宫内膜癌细胞增殖和侵袭转移等生物学行为调控作用的影响。方法收集子宫内膜癌组织标本20例、增生子宫内膜组织标本20例、正常子宫内膜组织标本10例,采用RT-PCR检测lncRNAHOTAIR在正常子宫内膜组织、增生子宫内膜组织及各期子宫内膜癌组织中的差异表达;利用脂质体Lipofectamine 2000将HOTAIR-siRNA转染Ishikawa细胞;分别采用MTT实验检测各组细胞增殖能力、Transwell小室实验对各组细胞迁移和侵袭能力进行检测。结果与正常子宫内膜组相比,lncRNA-HOTAIR在各期子宫内膜癌组的基因表达水平显著升高(P<0.05);lncRNA-HOTAIR在单纯性增生子宫内膜组、不典型性增生子宫内膜组的表达水平,差异无显著性(P>0.05)。HOTAIR-siRNA靶向抑制组的细胞增殖水平、侵袭能力与迁移能力显著低于空白对照组与阴性对照组(P<0.05)。结论 lncRNA-HOTAIR参与子宫内膜癌的发生、发展,可能在子宫内膜癌的转移侵袭中起重要作用。

Purpose To investigate the influence of long chain non-coding RNA （IneRNA）-HOTAIR on endometrial cancer cell proliferation, invasion, metastasis and other biological behaviour. Methods 20 cases of endometrial carcinoma tis- sue specimen, 20 cases of hyperplasia tissue sample and 10 ca- ses of normal tissue specimen were collected. Difference expression of lncRNA-HOTAIR in normal endometrium, hyperplasia endometrium tissue and endometrial carcinoma tissue at all periods were detected with RT-PCR assay. HOTAIR-siRNA transfection into Ishikawa cells was utilized with Lipofectamine 2000. MTF experiment was used to detected the proliferation ability of cells in all groups. Transwell chamber experiment was used to test the migration and invasion ability of cells in all groups. Resuits The gene expression level of of lncRNA-HOTAIR in en-dometrial carcinoma group at all stages was prominently increased compared with normal endometrium group （ P 〈 0. 05 ）. The expression level of lncRNA-HOTAIR in simple hyperplasia endometrium group and atypical hyperplasia endometrial group was not significantly different （ P 〉 0. 05 ）, Cell proliferation, invasion ability and migration ability of HOTAIR-siRNA targeting suppression group were lower than the blank control group and the negative control group significantly （ P 〈 0. 05 ）. Conelusion lncRNA-HOTAIR may involved in occurrence and de- velopment of endometrial cancer, which may play an important role in the aggression and metastasis of endometrial cancer.