nisin及大黄复方对子宫内膜炎大鼠血清中MMP-2和MMP-9活性的影响

Effects of nisin and rhubarb compound on the activity of serum MMP-2 and MMP-9 in the endometritis of rats

为研究乳酸链球菌素(nisin)及大黄复方对患大鼠子宫内膜炎血清中基质金属蛋白酶-2、基质金属蛋白酶-9(MMP-2、MMP-9)的作用,构建大鼠子宫内膜炎模型,将280只SD大鼠随机分为nisin组、低、中、高剂量大黄复方组、阳性对照组、生理盐水组以及模型组,建子宫内膜炎模型前0h和建模后24、48、72h,用ELISA试剂盒检测各试验组中基质金属蛋白酶(MMPs)和基质金属蛋白抑制物(TIMPs)的变化情况来确定治疗效果。结果表明:1)在0~72h之间,模型组中,MMP-9和TIMP-1的质量浓度显著低于生理盐水组(P<0.05),且MMP-2和TIMP-2的质量浓度逐渐降低;2)nisin和高剂量大黄复方组中MMP-9、TIMP-1、MMP-2和TIMP-2的质量浓度均显著高于生理盐水组(P<0.05),其中以nisin组和高剂量大黄复方组效果最佳。由此可见,nisin和高剂量大黄复方能显著提高子宫内膜炎大鼠血清中TIMP-1和TIMP-2的表达,抑制MMP-9和MMP-2的活性而发挥调控子宫内生理机能的作用,表明nisin、中、高剂量大黄复方对MMP-9、TIMP-1和MMP-2的质量浓度具有正调控作用。

Aim of this study was to investigate the influence of Streptococcus thermophilus （nisin） and rhubarb compound on the activity of serum matrix metalloproteinases （MMP）-2 and MMP-9 in the endometritis of rats.The endometritis model of rat was constructed,and a total of 280 SD rats were randomly divided into nisin group,low,medium and high doses of rhubarb compound groups,antibiotic group,saline group and model group.Models of endometritis were constructed at 0 h and modeling after 24,48,72 h,and the changes of the experimental group of matrix metalloproteinases （MMPs） and tissue inhibitor metalloproteinases （TIMPs） were detected by ELISA to determine the therapeutic effects.The results showed that：1） From 0 to 72 h,the concentration of MMP-9 and TIMP-1 in the model group was significantly lower than those in the normal saline group （P〈 0.05） and the concentration of MMP-2 and TIMP-2 decreases gradually;2）In the nisin and high dose of rhubarb compound group,the concentration of MMP-9,TIMP-1,MMP-2 and TIMP-2 were significantly higher than those in the normal saline group （P〈0.05）,in which the nisin group and high dose of rhubarb compound group showed the best effects.In conclusion,nisin and high dose of rhubarb compound significantly improved the expression in serum of TIMP-1 and TIMP-2 in endometritis of rats,inhibited the activity of MMP-9 and MMP-2 and played the role of regulation for the womb physiological function,showing that the concentration of nisin,medium and high dose of rhubarb compound had a positive effect on the concentration of MMP-9,TIMP-1 and MMP-2.