干扰IGF-1R的表达对口腔鳞状细胞癌增殖、分化及凋亡的影响研究

The effect of IGF-1R expression on the proliferation,differentiation and apoptosis of oral squamous cell carcinoma

目的探讨干扰IGF-1R的表达对口腔鳞状细胞癌增殖、分化及凋亡的影响。方法 Western blot检测IGF-1R在口腔鳞状细胞癌中的表达;将NC-siRNA、IGF-1R-siRNA转染口腔鳞状细胞癌CAL27细胞,未转染任何siRNA作为对照组,细胞转染48 h后,Western blot检测IGF-1R、Id-1、c-myc、Cleaved caspase3、p-AKT、p-m TOR蛋白表达;CCK8实验检测细胞增殖;流式细胞仪检测细胞凋亡。结果 IGF-1R在口腔鳞状细胞癌中的表达显著高于癌旁组织(P<0.01);IGF-1R-siRNA组细胞中IGF-1R蛋白表达显著低于对照组(P<0.01);NC-siRNA组细胞存活率、细胞凋亡率及Id-1、c-myc、Cleaved caspase3、p-AKT、p-m TOR蛋白表达与对照组差异不显著(P>0.05),IGF-1R-siRNA组细胞存活率及Id-1、c-myc、p-AKT、p-m TOR蛋白表达显著低于对照组,细胞凋亡率及Cleaved caspase3蛋白表达显著高于对照组(P<0.01)。结论 IGF-1R在口腔鳞状细胞癌中高表达,抑制IGF-1R的表达能显著降低细胞增殖及分化,并促进细胞凋亡,其机制与AKT/m TOR信号通路调控有关。

Objective To investigate the effect of IGF-1R expression on the proliferation,differentiation and apoptosis of oral squamous cell carcinoma.Methods The expression of IGF-1R in oral squamous cell carcinoma was detected by Western blot; NC-siRNA and IGF-1R-siRNA were transfected into oral squamous cell carcinoma CAL27 cells,and not transfected with any siRNA as the control group; cells were transfected for 48 h,the expression of IGF-1R,Id-1,c-myc,Cleaved Caspase3,p-AKT,p-m TOR protein was detected by Western blot; cell proliferation was detected by CCK8 experiment; cell apoptosis were determined by flow cytometry.Results The expression of IGF-1R in oral squamous cell carcinoma was significantly higher than that in adjacent tissue（ P〈0.01）; IGF-1R protein expression in IGF-1R-siRNA group was significantly lower than the control group（ P〈0.01）; the cell survival rate,apoptosis rate and Id-1,c-myc,Cleaved Caspase3,p-AKT,p-m TOR protein expression in NC-siRNA group was no significant difference with control group（ P〉0.05）; the survival rate and Id-1,c-myc,p-AKT,p-m TOR protein expression in IGF-1R-siRNA group was significantly lower than the control group,the apoptosis rate and the expression of Cleaved Caspase3 protein was significantly higher than the control group（ P〈0.01）.Conclusion IGF-1R is highly expressed in oral squamous cell carcinoma,inhibiting the expression of IGF-1R can significantly reduce cell proliferation and differentiation,and promote cell apoptosis,and its mechanism is related to the regulation of AKT/m TOR signaling pathway.