摘要
香樟叶斑病是近年来荆州市香樟树Cinnamomum camphora的一种常发病害,褐色坏死斑点1~2 mm,常两个至多个相互连接形成不规则病斑,遍布于整个叶片,严重影响香樟的正常生长。根据柯赫法则对感病组织进行分离、接种、再分离,再对获得的病原菌进行形态观察和r DNA-ITS、TUB-2和GPDH基因序列分析,结果表明引起香樟叶斑病的病原菌为炭疽菌属Colletotrichum的果生刺盘孢C.fructicola和暹罗刺盘孢C.siamense,属于国内首次报道。
Leaf spot disease on Cinnamomum camphora occurred severely and frequently in Jingzhou, Hubei province in recent years. The disease had the brown necrotic spot with 1-2 micrometers in length, two or more spots connected to perform a big spot in irregular shape and diffused onto the whole leave. It was strongly harmful to the growth of camphor. Based on Kohn＇s rule,the author finished the isola- tion, inoculation and re-isolation for the infected tissues, and then observed the morphology of the isola- ted pathogen and conducted gene sequence analysis of rDNA - ITS, TUB - 2 and GPDH. The results showed that the pathogens were CoUetotrichum fructicola and C. Siamense, both of them were firstly re- ported in China.
作者
徐明珠
罗欢
周志雄
贾国庚
邓建新
李传仁
XU Mingzhu et al(College of Agriculture, Yangtze University, Jingzhou 434025, Chin)
出处
《中国森林病虫》
北大核心
2017年第4期21-25,28,共6页
Forest Pest and Disease
基金
国家自然科学基金项目(31400014)
长江大学青年人才基金项目(2015cqr17
2016cqr08)
关键词
香樟
叶斑病
病原菌
分离
鉴定
Cinnamomum camphora
leaf spot disease
pathogen
isolation
identification
