叶黄素酯/α-环糊精包合研究

Evaluation of α-cyclodextrin inclusion compound of lutein esters

目的:研究叶黄素酯与α-环糊精的包合性能。方法:紫外法测定包合行为并计算表观包合常数;研磨法制备包合物;溶解度为指标确定包合比;DTA、DR-FTIR、UV-vis DRS验证包合物;与β-环糊精和混合环糊精的包合作用比较,测定其溶解度、稳定性。结果:叶黄素酯与α-环糊精表观包合常数Ka为127 L/mol;包合比1∶5时溶解度最佳;α-环糊精使叶黄素酯稳定性增强,溶解度提高为叶黄素酯原料的6倍以上。结论:α-环糊精应用于叶黄素酯性能优于β-环糊精和混合环糊精。

Abstract：Object ：To study properties of α-cyclodextrin inclusion compound of lutein esters. Methods： Inclusion process was evaluated by UV spectroscopy and then binding constants was calculated, inclusion compound was prepared by trituration method,lutein solubility was used as the index to determine the appropriate inclusion ratio,inclusion compound was confirmed by differential thermal analysis, infrared diffuse reflection spectrum and UV-vis diffuse reflection spectrum, the solubility and the stability of α-cyclodextrin,β-cyclodextrin and mixed cyclodextrins inclusion compounds of lutein esters were compared. Results：inclusion constants of lutein esters/α-eyclodextrin was 127 L/tool, optimum inclusion mass ratio of lutein esters and α-cyelodextrin was 1：5, the solubility of α-cyelodextrin inclusion compound was improved by more 6 times that of lutein esters,the stability of inclusion compound was enhaneed.Conclusions：α-cyclodextrin is superior to β-eyclodextrin and mixed cyclodextrins in preparation of lutein esters inclusion complex.