二甲亚砜法破壁条件对法夫酵母虾青素提取效果的影响

Effects of disrupting conditions on extracting astaxanthin from Phaffia rhodozyma by DMSO method

为了评价二甲亚砜(DMSO)法破壁在法夫酵母虾青素提取中的应用价值,研究了提取溶剂、DMSO用量、破壁温度、料液比、浸提时间、浸提次数6个条件因子对破壁后法夫酵母虾青素提取的影响,并对破壁条件进行了优化。实验结果表明:丙酮为最理想的提取溶剂;各主要因素破壁温度、料液比和浸提时间对虾青素的提取量影响依次减小,单因素及正交实验得到法夫酵母中虾青素提取的最佳条件为:以丙酮为提取溶剂、DMSO用量1∶1.5、浸提2次、破壁温度45℃、料液比1∶20(w/v)、浸提时间40 min,优化后虾青素的提取量为4.42 mg/g。

In order to evaluate the possibility of extracting astaxanthin from Phaffia rhodozyma disrupted cell wall by DMSO method, influences of several factors such as type of solvent, dosage of DMSO, temperature, the ratio of solid to liquid, extraction time and extracting times on recovery of astaxanthin from Phaffia rhodozyma were studied and conditions for disrupting the yeast cell wall were optimized. Results showed that acetone was the optimal solvent for extracting astaxanthin. The effect significance of extraction temperature, the ratio of solid to liquid and extraction time were gradually decreased on recovery of astaxanthin.The optimal conditions obtained from single factor and orthogonal experiment were as follows： acetone as the extracting solvent, 1：1.5 dosage of DMSO,2 times of extraction,45℃ of extraction temperature, 1：20 of the ratio of solid to liquid and 40 min of extraction time. Under the optimal conditions, the astaxanthin recovery from Phaffia rhodozyma was 4.42 mg/g.