SPME和SDE结合GC-MS分析荷叶中挥发性成分

Characterisation of volatile components of Lotus leaves by HS-SPME and SDE coupled to GC-MS

分别采用同时蒸馏萃取(SDE)法和固相微萃取(SPME)法并结合气相色谱-质谱联用(GC-MS)技术分析荷叶的挥发性风味成分。SPME法选用3种萃取头(CAR/PDMS、DVB/CAR/PDMS、PDMS/DVB)与SDE法对定性结果进行比较分析。结果表明,SDE法所鉴定出的挥发性成分有97种,相对含量为94.86%,且以烃类物质为主;SPME(CAR/PDMS)法所鉴定出的挥发性成分有29种,相对含量为89.26%,以醇类为主;SPME(DVB/CAR/PDMS)法所鉴定出的挥发性成分有62种,相对含量为85.97%,以醛类为主;SPME(PDMS/DVB)法所鉴定出的挥发性成分有59种,相对含量为86.96%,以醛类为主。由此可见,在荷叶挥发性成分分析中,四种方法萃取到的挥发性成份种类数从多到少依次是,SDE、SPME(DVB/CAR/PDMS)、SPME(PDMS/DVB)、SPME(CAR/PDMS)。

The volatile flavor components of lotus leaves were extracted by solid phase microextraction （ SPME）, simultaneous distillation extraction （ SDE ）, which was analyzed by gas chromatography - mass spectrometry （ GC - MS ） .Solid phase microextraction（SPME） of three extraction heads （ CAR/PDMS, DVB/CAR/PDMS, PDMS/DVB） and simultaneous distillation extraction（SDE） were used to compare qualitative results.The results showed that there were 97 kinds of volatile components identified by SDE and the percent of total peak area was 94.86% and the major compound was alkane ,there were 29 kinds of volatile components identified by SPME（CAR/PDMS） and the percent of total peak area was 89.26% and the major compound was ketone,there were 62 kinds of volatile components identified by SPME （DVB/CAR/PDMS）and the percent of total peak area was 85.97% and the major compound was aldehyde, there were 59 kinds of volatile components identified by SPME （PDMS/DVB） and the percent of total peak area was 86.96% and the major compound was aldehyde.This showed that the most kind of Volatile components can be obtained by SDE, followed by SPME （ DVB/CAR/PDMS ）, SPME （ PDMS/DVB ）, SPME （CAR/PDMS）.