甘草硒多糖对CCl_4诱导的小鼠急性肝损伤的影响作用

Effect of glycyrrhiza selenium polysaccharide on CCl_4-induced injury in mice

目的:探讨甘草硒多糖对CCl_4诱导的小鼠肝损伤的影响作用。方法:采用CCl_4建立小鼠急性肝损伤模型,通过测定各组小鼠血清中TP、ALB、ALT、AST、ALP、γ-GT的含量及肝组织匀浆中MDA、GPX水平,并结合病理学HE染色,表明甘草硒多糖对小鼠急性肝损伤的影响。结果:中、高剂量甘草硒多糖与模型组比较可明显降低急性肝损伤小鼠血清中的AST(p<0.05)、ALP(p<0.05)及肝组织中的MDA(p<0.05)和GSH-Px(p<0.05)水平,高剂量组还可明显降低ALT(p<0.05)含量,改善CCl_4所致的病理组织损伤。结论:甘草硒多糖对CCl_4诱导的肝损伤有一定的保护作用,其机制可能是通过清除自由基而达到防治肝细胞损害的疗效。

Objective：To investigate the effect of glycyrrhiza selenium polysaccharide on acute liver injury induced by CCl4 in mice.Methods ：TP, ALB, ALT, AST, ALP, γ-GT levels in serum and GPX, MDA levels in liver tissues were examined. In addition, pathologic changes were examined by HE staining. Results ： Glycyrrhiza selenium polysaccharide of middle and high dose can reduce the degrees of AST （p 〈 0.05 ）, ALP （p 〈 0.05 ） in serum and MDA and GSH - Px （p 〈 0.05 ） levels in liver tissue, and the ALT level in serum were decreased in high dose group, which can improve CCl4 -induced pathological tissue damage.Conclusion：This study confirms licorice selenium polysaccharide on CCl4-induced acute liver injury has protective effect, the mechanism is likely to be reached by scavenging free radicals and prevent liver damage.