脂多糖对人鼻黏膜上皮细胞人β防御素2诱导表达的影响

Roles of lipopolysaccharide in the expression of human β defensin 2 in human nasal mucosa epithelial cells

目的观察脂多糖(LPS)诱导人鼻黏膜上皮细胞人β防御素2(h BD-2)表达的影响。方法用LPS刺激人鼻黏膜上皮细胞,反转录聚合酶链反应(RT-PCR)检测h BD-2 mRNA的表达,免疫细胞化学检测h BD-2蛋白的表达。结果 LPS刺激2 h后人鼻黏膜上皮细胞可见h BD-2 mRNA的表达,并呈剂量和时间依赖性;刺激4 h后人鼻黏膜上皮细胞胞质中可见h BD-2蛋白的表达。结论一定剂量的LPS可诱导人鼻黏膜上皮细胞h BD-2的表达,这种表达具有一定的剂量和时间依赖性。

Objective To explore the role of lipopolysaceharide （LPS） in the expression of human β defensin 2 （hBD-2） in human nasal mucosa epithelial cells. Methods After the human nasal mucosa epithelial cells were stimulated with LPS, the expression of hBD-2 mRNA was detected by reverse transcription polymerase chain reaction （RT-PCR） and the expression of hBD-2 protein was deteeted by immunoeytochemistry. Results The hBD-2 mRNA could be detected 2 hours after LPS stimulation and expressed in a dose- and time-dependent manner. The hBD-2 protein could be detected in cytoplasm 4 hours after LPS stimulation. Conclusions A certain dose of LPS could induce the expression of hBD-2 in human nasal mueosa epithelial cells in a dose- and time-dependent manner.