丹红注射液对缺血性脑卒中大鼠药理作用机制影响研究

Effect of dan hong injection on pharmacological action mechanism of ischemic stroke in rats

目的探讨丹红注射液对缺血性脑卒中大鼠药理作用机制影响。方法 48只大鼠随机分为假手术组、模型组、阳性组、实验组,每组12只。假手术组和模型组给予等剂量生理盐水静脉注射,阳性组给予静脉注射尤瑞克林,实验组给予静脉注射丹红注射液,均为每日1次,连续7 d。比较各组大鼠一般活动情况,HE染色病理组织变化及血脂水平、血液流变学、神经肽Y(NPY)、强啡肽(Dyn)、神经功能缺损评分变化。结果阳性组和实验组总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)水平低于模型组,而高密度脂蛋白胆固醇(HDL-C)水平高于模型组(P<0.05);实验组TC、TG、LDL-C水平低于阳性组,而HDL-C高于阳性组(P<0.05);阳性组和实验组全血黏度高切、全血黏度低切水平低于模型组(P<0.05);实验组全血黏度高切、全血黏度低切水平低于阳性组(P<0.05);阳性组和实验组血浆NPY、Dyn水平低于模型组(P<0.05);实验组血浆NPY、Dyn水平低于阳性组(P<0.05);阳性组和实验组神经功能缺损程度评分低于模型组(P<0.05);实验组神经功能缺损程度评分低于阳性组(P<0.05)。结论丹红注射液可明显改善缺血性脑卒中大鼠血脂、血液流变学及神经功能,降低血浆NPY、Dyn水平,值得临床进一步研究。

Objective To investigate the effect of dan hong injection on pharmacological action mechanism of ischemic stroke in rats.Methods 48 rats were randomly divided into sham group （n=12）,model group （n=12）,positive group （n=12）,the experimental group （n=12）.Operation group and model group were received normal saline intravenously.Positive group were given intravenous Kallikrein and experimental group were given intravenous injection danhong,both of which once daily,continuous for 7 d.The general activities,staining pathological changes and disease,lipid levels,blood rheology,NPY,Dyn,neurological impairment score were compared.Results The levels of TC,TG,LDL-C of positive and experimental groups were lower than the model group while the HDL-C levels was higher （P〈0.05）.In experimental group,the level of TC,TG,LDL-C were lower than positive group,while HDL-C higher than the positive group （P〈0.05）.positive group and experimental group high shear whole blood viscosity,whole blood viscosity at low shear level lower than the model group （P〈0.05）;In experimental group,high shear whole blood viscosity and low shear whole blood viscosity at were lower than positive group （P〈0.05）.The level of plasma NPY,Dyn of positive and experimental groups were lower than the model group （P〈0.05）.The plasma NPY,Dyn level of experimental were lower than the positive group （P〈0.05）.The neurologic deficit score of positive and experimental groups were lower than that of the model group （P〈0.05）.The impairment score of experimental group,neurological were lower than positive group （P〈0.05）.Conclusion Dan hong injection can significantly improve ischemic stroke serum lipids,blood rheology and nerve function,reduce plasma NPY,Dyn,which is worth studying.