β-catenin在肝癌组织中的表达及对肝癌患者预后的影响

β-catenin expression in hepatocellular carcinoma and its effect on prognosis

目的:探讨β-catenin在肝细胞癌(HCC)组织中的表达及其与肝癌预后的关系。方法:用免疫组织化学方法检测83例HCC、26例癌旁组织和5例正常肝组织中β-catenin蛋白的表达情况,并对行肝癌根治性术患者进行术后随访,分析β-catenin蛋白表达与临床因素、HCC病理分化程度及患者术后生存的关系。结果:β-catenin蛋白在HCC、癌旁组织及正常肝组织中的阳性表达率分别为68.67%(57/83)、30.77%(8/26)和20%(1/5),其在HCC中的表达阳性率高于正常肝组织及癌旁组织(P<0.05);β-catenin阳性表达率与肝癌病理分化程度呈负相关(r=-0.34,P=0.027);有效随访68例,在随访的5年内,β-catenin阳性表达组平均生存时间11.2个月,β-catenin阴性表达组平均生存时间26.6个月,Kaplan-Meier生存分析表明β-catenin阴性表达患者比阳性表达患者术后生存更长,Log-Rank检验两组预后有显著性差异(χ~2=15.138,P=0.000)。结论:β-catenin与HCC分化程度相关,β-catenin在肝癌组织中的高表达提示患者预后不良。

OBJECTIVE：To detect the expression of β-catenin and its prognostic significance in hepatocellular carcinoma.METHODS：The expression of β-catenin protein was detected by immunohistochemistry in83cases of resected HCC tissues,26cases of adjacent tissues and5cases of normal liver tissues. The integrated follow-up data were rerospectively analyzed in patients who had undergone radical hepatectomy. The relationships betweenβ-catenin and clincal variables,HCC pathological differentiation and postoperative survival were statistically analyzed.RESULTS：The positive expression rates of β-catenin in the HCC tissues,adjacent tissues and normal liver tissues were 68.67% （57/83）、30.77%（8/26） and 20%（1/5）. The positive rate of β-catenin in HCC was significantly higher than that of adjacent tissues and normal liver tissues （P＆lt;0.05）. The positive expression rates of β-catenin was negatively correlated with pathological differentiation （r=-0.34,P=0.027）. In the 83 follow-up data,68 cases were effective. In the first 5 years of follow-up data,and among patients with positive expression of β-catenin,the mean survival time was 11.2 months. Among patients with negative expression of β-catenin,the mean survival time was 26.6 months. Kaplan-Meier survival analysis revealed that patients with negative expression of β-catenin had longer survival time and their prognosis was 2significantly different based on Log-rank test（χ2=15.138,P=0.000）.CONCLUSION：Expression of β-catenin was correlated with pathological differentiation in HCC and its high expression predicted poor prognosis.