LIN28A、E-cadherin和vimentin在浸润性乳腺癌中的表达及其临床意义

Expressions of LIN28A,E-cadherin and vimentin in invasive breast cancer

目的:研究RNA结合蛋白LIN28A、上皮间质转化相关因子E-cadherin和vimentin在浸润性乳腺癌中的表达及三者的相关性,探讨其对乳腺癌进展的影响。方法:收集40例浸润性乳腺癌组织标本及其对应的癌旁组织,实时荧光定量PCR(qPCR)和免疫组织化学法分别检测LIN28A、E-cadherin和vimentin的mRNA和蛋白的表达,分析其与临床病理指标之间的关系,TUNNEL法检测LIN28A阳性癌组织中的细胞凋亡情况,统计分析LIN28A和EMT相关蛋白之间的相关性及其与乳腺癌患者生存率之间的关系。结果:qPCR结果显示,LIN28A mRNA在乳腺癌组织中的相对表达水平高于癌旁组织,其与E-cadherin mRNA的表达水平呈负相关,与vimentin mRNA呈正相关(r依次=-0.340和0.380,P均<0.05)。免疫组织化学检测结果显示,在乳腺癌组织中,LIN28A、Ecadherin和vimentin蛋白表达率分别为60%、55%和65%。LIN28A蛋白的表达与E-cadherin蛋白呈负相关(r依次=-0.533和0.364,P均<0.05),与vimentin蛋白呈正相关。LIN28A蛋白表达与肿瘤大小、病理分级和淋巴结转移有关。TUNEL检测结果显示,LIN28A蛋白表达阳性的乳腺癌组织中凋亡细胞率低于与癌旁组织(P<0.05)。Kaplan-Meier生存分析显示,乳腺癌组织LIN28A蛋白表达阳性患者的生存率低于LIN28A蛋白表达阴性者(P<0.05)。结论:RNA结合蛋白LIN28A可能通过EMT促进乳腺癌的进展和转移,并且LIN28A高表达可能影响乳腺癌患者的预后。

OBJECTIVE：To investigate the expression of RNA-binding proteins LIN28A,epithelial-mesenchymal transition （ EMT） - related p roteins E-cadherin and vimentin in invasive breast cancer and to understand their role ind evelopment of invasive breast cancer.METHODS：Quantitative real-time PCR （qPCR） and immunohistochemical staining were used to detect expressions of LIN28A,E-cadherin and vimentin mRNAs and proteins in invasive breast cancer tissues and the corresponding adjacent tissues. Relationships between mRNA and protein expressions of LIN28A,E-cadherin and vimentin were analyzed. TUNNEL staining was used to detect apoptosis of invasive breast cancer tissues with high LIN28A expressions. The relationship between LIN28A and EMT-related proteins and survival rate of breast cancer patients were statistically analyzed.RESULTS：qPCR results show that the relative expression level of LIN28A mRNA in breast cancer tissues were higher than a djacent tissues, negatively correlated with E-cadherin mRNA,and positively correlated with vimentin mRNA（P〈0.05）. Immunohitochemistry staining results show that in breast cancer tissues,the expression rates of LIN28A,E-cadherin and vimentin protein were 60%, 55% and 65%,respectively. The expression of LIN28A protein was negatively correlated with E-cadherin protein,positively correlated w ith v imentin protein. LIN28A expression was correlated with tumor size,histological grade and lymph node metastasis. TUNNEL assay show that high expression of LIN28A could d ecrease a poptosis in invasive breast cancer（P〈0.05）. The survival rate of patients with positively expressed LIN28A was significantly lower than that of LIN28A negative patients （P〈0.05）. CONCLUSION：The RNA-binding protein,LIN28A,may promote the occurrence,development and metastasis of breast cancer via EMT,while high expression of LIN28A may affect prognosis of patients with breast cancer.