摘要
建立了一种高效液相色谱法快速、准确测定茶叶中氟。样品经浸泡提取后,以邻苯二甲酸为背景吸收,以Agilent ZORBAX-ODS柱(4.6mm×250mm,5μm),流动相为乙腈-0.1mol/L邻苯二甲酸溶液(15∶85,V∶V),柱温35℃,检测波长294nm,流速1.0m L/min的条件进行实验。结果表明:氟在0.5~10.0μg/m L范围内线性关系良好,平均回收率为101.0%。建立的方法准确、可靠,可以用于茶叶中氟的含量检测。
A quick and accurate High Performance Liquid Chromatography（ HPLC） method was established to detect the fluoride illegally added into tea. After extracted and absorbed by hot water,The sample was determined by HPLC and the background absorption was 1,2-Benzenedicarboxylic acid. The separation of fluoride was performed on a Agilent ZORBAX-ODS column（ 4. 6mm × 250 mm,5μm） using acetonitrile-0. 1mol/L 1,2-Benzenedicarboxylic acid（ 15∶85,v∶v） as the mobile phase with flow rate of 1. 0 m L/min. The temperature was35℃ and the detection was at 254 nm. The result showed that there is a good linear relation in the range of 0. 5 ~ 10. 0μg/m L with correlation coefficient of 0. 9998. The average recovery was 101. 0%,RSD = 1. 67%. The method is accurate and feasible,suitable for determination of fluoride in the tea.
出处
《中国食物与营养》
2017年第7期10-12,共3页
Food and Nutrition in China
