超高效液相色谱——串联质谱法同时测定水稻中丙草胺和西草净残留

Simultaneous determination of residue of pretilachlor and simetryn in paddy by UPLC-MS/MS

建立了超高效液相色谱—串联质谱仪测定稻田环境中的丙草胺和西草净的残留分析检测方法。糙米、稻壳、植株、土壤以及田水样品中的丙草胺和西草净以乙腈为提取剂,N-丙基乙二胺(PSA)净化,采用超高效液相色谱—串联质谱测定。丙草胺在0.01~5.0 mg/L范围内线性良好,相关系数为0.997 4,丙草胺在糙米、稻壳、植株、土壤以及田水中的平均加标回收率为71.8%~103.6%,相对标准偏差为1.7%~9.2%;西草净在0.005~2.5 mg/L范围内线性良好,相关系数为0.997 9,西草净在不同样品中的平均加标回收率为75.0%~107.3%,相对标准偏差在1.7%~11.9%之间。该方法重复性好,准确度、精密度均能满足农药残留量分析与检测的技术要求。

A method for the simultaneous determination of residue of pretilachlor and simetryn in rice filed environment by ultra - performance liquid chromatography tandem mass - spectrometry （ UPLC - MS/MS） was established. The residues in unpolished rice, rice husk, plant, soil and field water were extrac-ted by acetonitrile, purified by N - propyl Ethylenediamine. The residues of pretilachlor and simetryn in samples were determined by UPLC - MS/MS. Pretilachlor showed a good linear relationship in the range of 0.01 -5.0 mg/L with the correlation coefficient of 0- 9974 and the average recovery rates in unpol-ished rice,rice shell, plant,soil and field water were 7 1 .8% -103. 6% ; Simetryn showed a good linear relationship in the range of 0- 005 - 2. 5 mg/L with the correlation coefficient of 0- 997 9 ,and the average recovery rates in different samples were 75. 0% -107. 3% with their relative standard deviations of 1.7% - 9. 2% - The method has good repeatability, accuracy and precision, and can meet the technical requirements