哈茨木霉基因组中NRPS基因多样性的生物信息学分析

Bioinformatics Analysis of NRPS Gene Diversity in Trichoderma harzianum

为了解哈茨木霉中非核糖体肽的生物合成,本文通过本地BLAST、结构域分析结合抗生素和次生代谢产物分析软件(antiSMASH)、天然产物结构域查找软件(NaPDoS)等次生代谢产物生物合成基因分析工具对哈茨木霉基因组中的非核糖体肽合成酶NRPS基因进行分析,通过本地BLAST获得42个可能具有NRPS基因的重叠群(contig),开放阅读框及结构域分析显示具A-T-C(A为腺苷酰化结构域、T为肽酰基载体蛋白结构域,又称为PCP结构域、C为缩合结构域)3个结构域的蛋白序列有21条;antiSMASH分析结果显示其基因组中合成NRPs的基因簇有10个;NaPDoS和聚类分析结果表明哈茨木霉中的NRPS基因催化合成的NRPs包括铁载体类、毒素类、抗生素类及一些未知化合物。

We analyzed the genome of Trichoderma harzianum with genome mining, and using the biosynthesis gene analyzing tools such as antiSMASH, NaPDoS, in order to find out the NRPS gene and classified them. We obtained 42 NRPS gene contigs that might biothetases NRPSs, the analysis results of the open reading frame and domains indicated that there were 21 proteins which have A- T-C three domains; The outcome of antiSMASH showed that there were 10 contigs that can biosynthetase NRPSs in the genome of Trichoderma harzianum; The clustering analysis of NaPDoS indicated that the compund of NRPSs biosynthesis is Calcium-dependent antibiotics, toxins including HC- toxin, gliotoxin and microcystins, cyclosporine, and some unknown compound.