温度对南黄海定生绿藻光合生理影响的研究

A Study on the Effect of Temperature on the Photosynthetic Physiology of Attached Green Algae in the South Yellow Sea

本研究以南黄海定生绿藻为材料,在室内不同温度下进行培养,通过对叶绿素荧光等参数进行测定,研究了温度对其光合生理特征的影响.结果表明:采集定生绿藻形态特征与肠浒苔相似,该绿藻在低温条件下(5℃)藻体最大量子效率(Fv/Fm)、实际量子效率(ΔF/Fm')以及相对电子传递速率(rETR)等参数均显著低于其他处理组样品,而20℃下样品的上述叶绿素荧光参数均高于其他处理组.诱导曲线表明,在给定光照条件下,20℃下样品光化学猝灭(qP)最高,而5℃处理组最低,非光化学猝灭(NPQ)结果则相反,5℃处理组样品要显著高于其他温度处理.本研究结果说明,温度对定生绿藻的光合生理活性有较大影响,20℃下该绿藻光合生理活性活跃,而低温条件会明显抑制其光能的吸收利用,温度的进一步上升也会降低其光合活性.

In this paper, the authors cultured the attached green alga collected from the South Yellow Sea under different temperature, and studied the effect of temperature on the photosynthetic physiology characteristic of this green alga through the measurement of chlorophyll forescence parameters. The results indicated that the morphological characteristic of the attached alga was similar to that of Ulva intestinalis. The Fv/Fm, Δ F/Fm＇ and rETR of sample cultured under 5℃ were signifcantly lower than those of algae cultured under other four temperatures, and those chlorophyll fuorescence parameters of sample cultured under 20 ℃ were higher than other treatments. The measurement of induce curves also indicated that the photochemical quenching （qP） of the sample cultured under 20 ℃ was the highest and the sample under 5 ℃ was lowest in all temperature treatments, and that the non-photochemical quenching （NPQ） results were opposite to that of qP. And algae cultured under 5 ℃ showed the highest NPQ in all treatments. According to the research, temperature had a close relationship with the photosynthetic characteristics of the attached alga. Low temperatures obviously inhibited the light use efciency of sample, and 20 ℃ was optimal for photosynthetic physiology of the attached green alga, but when the temperature increased much higher, the photosynthetic activity also decreased.