微透析采样考察玉龙散中乌头碱在大鼠皮肤局部的透皮吸收过程

Pharmacokinetics of Aconitine in Rats Treated with Yu Long San by Microdialysis

目的:考察玉龙散中乌头碱的透皮吸收过程。方法:采用HPLC-MS/MS技术建立SD大鼠皮肤微透析液中乌头碱含量的测定方法。通过考察微透析技术中对乌头碱体内回收率的影响因素,确定采样条件。经皮给药后,考察乌头碱的局部透皮吸收过程。结果:本实验成功建立了HPLC-MS/MS测定微透析样品中乌头碱含量的方法。微透析采样流速选择1.5μL·min^(-1),采样间隔为30 min。乌头碱的AUC为18 973.27 h·ng·mL^(-1),MRT为14.97 h,C_(max)为2 976.38 ng·mL^(-1),T_(max)为11.76 h。结论:微透析采样技术可用于玉龙散中乌头碱的透皮吸收过程研究。

Objective：To investigate the pharmacokinetics of aconitine in rats treated with Yu Long San by microdialysis.Methods：The suitable microdialysis sampling technical conditions of aconitine were developed by investigating the velocity，the concentration of aconitine and other factors.The rat plasma samples were collected by microdialysis sampling technique.The concentration of aconitine was determined by LC-MS.Results：The suitable conditions of microdialysis for ephedrine were the velocity of 1.5 μL·min^-1，sample interval for 30 min.The AUC was 18 973.27 h×ng·mL^-1，MRT was 14.97h，Cmax was 2 976.38 ng·mL^-1，Tmax was 11.76 h.Conclusion：Microdialysis is suitable for studying the pharmacokinetics of aconitine in rats treated with Yu Long San.