摘要
目的:建立金银花的UPLC指纹图谱。方法:以绿原酸为参照物,采用Acquity UPLC^(TM)BEH C_(18)色谱柱(2.1 mm×50 mm,1.7μm);流动相为乙腈-0.1%甲酸,梯度洗脱;流速为0.3 mL/min;柱温40℃。对金银花及其次生代谢物的色谱图进行分析,并利用《中药色谱指纹图谱相似度评价软件》(2004A)对二者共有峰的相似度进行质量评价。结果:建立了10批金银花的UPLC指纹图谱的共有模式,特征图谱有10个共有峰,以对照品绿原酸为对照,各色谱峰有较好的分离效果。结论:本方法快速、高效,可用于金银花药材的质量控制。
Objective ： To establish the fingerprint spectrum of Flos lonicerae by HPLC technique. Methods： With chlorogenie acid as reference, the fingerprint was established on Acquity UPLC^TMBEH C18Column （2.1mm×50mm,1.7μm）. Acetonitrile - 0.1% formic acid was used as gradient mobile phase at the flow rate of 0. 3 mL/min. The column temperature was 40℃. The ehromatograms of Flos lonicerae and its secondary metabo- lites were analyzed, and its quality was evaluated by Chinese Medicine Chromatographic Fingerprint Evaluation System （2004 A ）. Results： Common mode of the fingerprints was established from 10 batches of Flos lonicerae. There were total 10 common spectrum peaks taking ehlonogenic acid as reference, which were iden- tified with better separation. Conclusion ： The method is time - saving, efficient and can be used for the quality control of Flos lonicerae.
作者
石朗
王丹丹
杨丽军
李杉杉
刘健
陈大忠
SHI Lang WANG Dan - dan YANG Li - jun LI Shan - shan LIU Jianl CHEN Da - zhong(The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150040, China Heilongfiang University of Chinese Medicine, Harbin 150040, China)
出处
《中医药信息》
2017年第4期26-28,共3页
Information on Traditional Chinese Medicine
基金
黑龙江省中医药科研项目(No.ZHY16-099)
黑龙江省应用技术研究与开发计划项目(No.GC13C109)
关键词
金银花
次生代谢物
绿原酸
UPLC
指纹图谱
Flos lonicerae（ Jinyin Hua）
Secondary metabolites
Chlorogenic acid
UPLC
Fingerprint
