建立凤尾草中总黄酮和木犀草素的含量测定方法

Development and validation for the determination of total flavonoids and luteolin from Pteris multifida Poir

目的 建立凤尾草中木犀草素及总黄酮的含量测定方法.方法 采用D101大孔树脂分段洗脱,并采用紫外分光光度法测定总黄酮含量;采用HPLC法测定木犀草素含量.色谱柱为RP-C18柱（4.6 mm×250 mm,5μm）;流动相为乙腈-0.2%磷酸（35：65）,检测波长349 nm,流速1.0 ml/min,柱温30℃.结果 3批凤尾草药材中木犀草素含量依次为0.015%、0.019%、0.016%;总黄酮含量依次为0.55%、0.66%、0.59%.结论 所建立的分析方法准确、简便,可为定量评价凤尾草药材的质量提供依据.

Objective To determine the content of total flavonoids and luteolin from Pteris multifida Poir. Methods The content of total flavonoids was determined by gradient elution of macroporous resin D101 and ultraviolet spectrophotometry. The content of luteolin was determined by HPLC. The analysis was performed on a RP-C18 column（4.6 mm×250 mm, 5 μm） with aceconitrile-0.2% phosphoric acid （35：65） as the mobile phase at a flow rate of 1.0 ml/min, and 30 ℃ temperature. Results The detection of wave length was set at 349 nm. The content of luteolin was 0.015%, 0.019%, 0.016%, and the content of total flavonoids was 0.015%, 0.019%, 0.016%, respectively. Conclusions The method is suitable for the determination of flavonoids componets from Pteris multifida Poir.