用微核试验来评价复方银耳制剂的抗辐射作用

The Application of Micronucleus Test for the Evaluation of Antiradiation Protective Effect of Tremella Compound

本文采用细胞遗传学测试方法之一微核试验(Mt),来评价一种抗辐射新药复方银耳制剂(下称银参饮)对受1Gy γ线急性照射狗的辐射防护作用。实验分银参饮高、低剂量、银耳、胱胺及照射对照五个组。结果显示:服用银参饮具有良好的抗辐射作用,其抗放效价类似于胱胺而明显高于银耳组。各组的剂量减低系数(DRF)分别为:银参饮高剂量组2.01、银参饮低剂量组1.72,胱胺组1.55、银耳组1.11。

Micronucleus test was applied for the evaluation of the effect of a new radioprotective drug-tremella compound or Yin Shen Yin on the whole body gamma irradiated dogs. Twenty-five male dogs were divided into 5 groups: the tremella gorup, the mercaminum group, the Yin Shen Yin high dosage group, the Yin Shen Yin low dosage group and the control group. Before exposure to 1 Gy gamma irradiation, the dogs were given the aggents continually for 10 days. The measurement of micronucleus frequencies in PHA-activated lymphocytes was made as an evaluation indicator of antiradiation protection effect. Results showed that the micronucleus frequencies in the 2 Yin Shen Yin groups were significantly lower than those of the tremella and control groups, but they were similar to that of the mercaminum group. And the DRFs of the groups were: the Yin Shen Yin high dosage group was 2.01, the Yin Shen Yin low dosage group 1.72, the mercaminum group 1.55 and the tremella group 1.11.