非对称场流分离技术在蛋黄浆质低密度脂蛋白粒径表征中的应用

Application of asymmetrical flow field-flow fractionation for size characterization of low density lipoprotein in egg yolk plasma

通过自组装的非对称场流分离系统(AF4)与紫外可见光检测器联用分离表征了笼养鸡蛋、柴鸡蛋、鹌鹑蛋和鸭蛋蛋黄浆质中的低密度脂蛋白(LDL)。在近似蛋黄浆质生理条件下,研究了进样量、交叉流流速、膜的类型对AF4蛋黄浆质中LDL分离表征的影响;考察了该方法的精密度。在优化的AF4分析条件下,检测出了笼养鸡蛋、柴鸡蛋、鹌鹑蛋和鸭蛋蛋黄浆质中LDL的水力学粒径分布。LDL的AF4洗脱峰高和峰面积的日内精密度分别为1.3%和1.9%(n=7),日间精密度分别为2.4%和2.3%(n=7)。研究结果表明,该方法可用于分离禽类蛋黄浆质中的LDL,同时能够得到LDL水力学粒径分布。

Home-made asymmetrical flow field. flow fractionation (AF4) system, online cou. pled with ultraviolet/visible (UV/Vis) detector was employed for the separation and size characterization of low density lipoprotein (LDL) in egg yolk plasma. At close to natural condition of egg yolk, the effects of cross flow rate, sample loading, and type of membrane on the size distribution of LDL were investigated. Under the optimal operation conditions, AF4. UV/Vis provides the size distribution of LDL. Moreover, the precision of AF4. UV/Vis method proposed in this work for the analysis of LDL in egg yolk plasma was evaluated. The intra. day precisions were 1.3% and 1.9% (n = 7) and the inter. day precisions were 2.4% and 2.3% (n = 7) for the elution peak height and elution peak area of LDL, respectively. Results reveal that AF4. UV/Vis is a useful tool for the separation and size characterization of LDL in egg yolk plasma.